Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165801_dq4o TTTGATACAAGCTAGAGTAATCTGAGAAGAGG(+AAAAAA)GATCCTCAATTGAAAAACTGCCTACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240606165800_2vly TAATCCCAGCTCCTAGGAGGCAGGGACAAGTG(+AAAAAA)GATCCCTGTGAGTTAGAGACTAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606165759_zf1z TATGTAGACCAGGCTAGTCTCTAACTCACAGG(+AAAAAA)GATCCACTTGTCCCTGCCTCCTAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606165758_5xvk GAAGAGAGACAAGGAGAAGCAGAAAGCAGAAG(+AAAAAA)GTACCCTTTTATTGGCATTTTAACAATGGCA NGG 10 8 17 8 24 0 100 5 10
    240606165756_4z25 CGTGCTGCCATTGTTAAAATGCCAATAAAAGG(+AAAAAA)GTACCTTCTGCTTTCTGCTTCTCCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606165755_8i6b TCTTTTTGTAAGGTTCTGAGACTTTATGGAAG(+AAAAAA)GTACCTCTGCATTCTCATAAACCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240606165754_2jlt GGATCAGAGTCAGGGTTTATGAGAATGCAGAG(+AAAAAA)GTACCTTCCATAAAGTCTCAGAACCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240606165752_2iaa AACAGCCGTGGGCGGTGGGTGTTGTTACTGTG(+AAAAAA)GTACCTACCATGCCATCTTAACCGAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240606165751_cw3q AGGTCTTGGTTTCGGTTAAGATGGCATGGTAG(+AAAAAA)GTACCACAGTAACAACACCCACCGCCCACGG NGG 10 8 17 8 24 0 100 5 10
    240606165749_6sx6 AGGTCTACTTTGGGTCATTATTTCACCTCAGG(+AAAAAA)GATCCATCTCTCTGCCTTATGCTCCAGTTTT NGG 10 8 17 8 24 0 100 5 10