Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163446_omri GTCCAGTAATATTCAGTCATAGCAGGCCTCAG(+AAAAAA)GATCCGATTGTTGGATTAAGTAGTCGAGATT NGG 10 8 17 8 24 0 100 5 10
    240606163445_b7xi GCTCTAAGAGGAAAGCTGTATTTTGACTTGTG(+AAAAAA)GATCCTGGTCCACAGGAGGCAACACACCATG NGG 10 8 17 8 24 0 100 5 10
    240606163443_w39p CCTGGCATGGTGTGTTGCCTCCTGTGGACCAG(+AAAAAA)GATCCACAAGTCAAAATACAGCTTTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606163441_5eah AATAATGCTACATTGAACAGGGATCGGGGTTG(+AAAAAA)GTACCTGTAAACATTGATCCCACTGCATCCG NGG 10 8 17 8 24 0 100 5 10
    240606163440_jek9 CGTAACGGATGCAGTGGGATCAATGTTTACAG(+AAAAAA)GTACCAACCCCGATCCCTGTTCAATGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606163439_52sf TAAGGTAGCCCTGGTCTGGAATTTTGAAACAG(+AAAAAA)GATCCAAGCAAGTGTCTCCTTCATTTGCACA NGG 10 8 17 8 24 0 100 5 10
    240606163438_7jk4 AGCTGTGTGCAAATGAAGGAGACACTTGCTTG(+AAAAAA)GATCCTGTTTCAAAATTCCAGACCAGGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606163436_35xd TGATTTACCTTCAGTGTCCTGGGTGAGGTGGG(+AAAAAA)GTACCATCATTATGACCCAGGAAAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240606163434_yvkg TACCGTGTCCCTTTTCCTGGGTCATAATGATG(+AAAAAA)GTACCCCACCTCACCCAGGACACTGAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606163433_y6ht GTGTGTCATTGCCCTCCCCTGTGGTCATCTGG(+AAAAAA)GTACCCCTGCCCATCCATTGTTTTTAGAACA NGG 10 8 17 8 24 0 100 5 10