Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164636_my6l AGCTCTCTGAAGAAGATTCATAAGGGCCTGGG(+AAAAAA)GATCCATATTCCCTTTGGTCTCTTGGTTACA NGG 10 8 17 8 24 0 100 5 10
    240606164634_5xei CACTGCTTTCAGGGCCAGATTAAAGCCTTATG(+AAAAAA)GATCCAACCCTGGGTTCCCGGGAGAACCCAT NGG 10 8 17 8 24 0 100 5 10
    240606164633_d9q8 TGCAGATGGGTTCTCCCGGGAACCCAGGGTTG(+AAAAAA)GATCCATAAGGCTTTAATCTGGCCCTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606164631_2uaw AGGATGCTTTCTTTGGCTGAGGCATCTCCCTG(+AAAAAA)GTACCAGCCCTGTACTCTTTCCCTCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240606164629_g0wn CCAGCATCATGGAGGGAAAGAGTACAGGGCTG(+AAAAAA)GTACCAGGGAGATGCCTCAGCCAAAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606164627_lvqa ATGGATCATGGCCCAGGAGAGCTGCAGGCCTG(+AAAAAA)GATCCAGGGTGGCCAGGATGGAATACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606164625_ujpd ACCAAAACCTGTATTCCATCCTGGCCACCCTG(+AAAAAA)GATCCAGGCCTGCAGCTCTCCTGGGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240606164623_veeo AGATGTGATCTTGCCAAAGCCATTAGACCTTG(+AAAAAA)GTACCCAGGCCCTCCTATAATGAGCTGATTG NGG 10 8 17 8 24 0 100 5 10
    240606164621_2ej3 GCAGACAATCAGCTCATTATAGGAGGGCCTGG(+AAAAAA)GTACCAAGGTCTAATGGCTTTGGCAAGATCA NGG 10 8 17 8 24 0 100 5 10
    240606164620_a9od TCTGTGTACCATTTGAATGCCTGGCACTGAGG(+AAAAAA)GATCCCCTGAAATAAGGCTATAGAGGGTTGT NGG 10 8 17 8 24 0 100 5 10