Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163431_zs4u AGGGTTGTTCTAAAAACAATGGATGGGCAGGG(+AAAAAA)GTACCCAGATGACCACAGGGGAGGGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240606163429_1avu CTCATCGCCTTTTCTTCTAGACCTCCTTCAGG(+AAAAAA)GATCCGTGAAATGAAAAGAGAAACAGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606163428_r2jx CCTGGGGCACCTGTTTCTCTTTTCATTTCACG(+AAAAAA)GATCCCTGAAGGAGGTCTAGAAGAAAAGGCG NGG 10 8 17 8 24 0 100 5 10
    240606163427_mm4k CCATCAGTTGTTTTAACAAACGTAGGTGATCG(+AAAAAA)GTACCTAGAAACTGTTATTTCAGTTAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606163426_7hrh TTGTCATGTCTAACTGAAATAACAGTTTCTAG(+AAAAAA)GTACCGATCACCTACGTTTGTTAAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240606163425_3wmb CTGATGCCTGTGGAGGCCAGGAGTAAATGTTG(+AAAAAA)GATCCTTTGGAACTAGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606163423_8pa1 ACTCACAACTGTCTGTAACTCTAGTTCCAAAG(+AAAAAA)GATCCAACATTTACTCCTGGCCTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606163422_ege1 CTGGACACACAGAGTCAAAGTAGGAAAACATG(+AAAAAA)GATCCCTGAGACCTTCACAGCGACAGACTAG NGG 10 8 17 8 24 0 100 5 10
    240606163420_9nxn CTGTGCTAGTCTGTCGCTGTGAAGGTCTCAGG(+AAAAAA)GATCCATGTTTTCCTACTTTGACTCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606163419_19xj CTTTAAAGTATTTCATAATACCTTGAGGATGG(+AAAAAA)GATCCAATTCTGAACACAAGGTCCGCTTATA NGG 10 8 17 8 24 0 100 5 10