Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160715_efsw TCTGTGGACCAGGCTGGCCTTGAACTCACAGG(+AAAAAA)GATCCGCCTGCCTCTGCCTCCCAATTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606160713_dwcc TAATCCCAGCAATTGGGAGGCAGAGGCAGGCG(+AAAAAA)GATCCCTGTGAGTTCAAGGCCAGCCTGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606160711_3k9x GACCGACTCCATTCTTGAACGAGACGGGGCAG(+AAAAAA)GATCCTCTCGATGTTGCAAATAACGCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606160710_th79 ATGGTTTGCTGCGTTATTTGCAACATCGAGAG(+AAAAAA)GATCCTGCCCCGTCTCGTTCAAGAATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606160708_opu1 CCAACCTGCCCAGCTAGACCCTTATTGTGCCG(+AAAAAA)GATCCCCAACTTATTCACTGCAGAGCTGCCG NGG 10 8 17 8 24 0 100 5 10
    240606160706_a5pn ACTCGCGGCAGCTCTGCAGTGAATAAGTTGGG(+AAAAAA)GATCCGGCACAATAAGGGTCTAGCTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606160705_ma28 ACAGCTCTTAGCTCTTAACCCTTGGGTTCCAG(+AAAAAA)GATCCACTCCATTATAGTAAGGGAGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606160704_bp6t TTGATGGGAGACTCCCTTACTATAATGGAGTG(+AAAAAA)GATCCTGGAACCCAAGGGTTAAGAGCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606160701_052j TGGACTCCCACCAGCAATGGAGTGTTCCCCTG(+AAAAAA)GATCCATATCTTCGTCAGCACGAGCTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606160659_gotz CTCAAGCAACAGCTCGTGCTGACGAAGATATG(+AAAAAA)GATCCAGGGGAACACTCCATTGCTGGTGGGA NGG 10 8 17 8 24 0 100 5 10