Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160612_cia6 TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+AAAAAA)GTACCTGCACTTGACAGGGCCTGGCACATGG NGG 10 8 17 8 24 0 100 5 10
    240606160611_nkoy ACTCCCCATGTGCCAGGCCCTGTCAAGTGCAG(+AAAAAA)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606160610_2glf TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+AAAAAA)GTACCTGCACTTGACAGGGCCTGGTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606160609_75fw ACTCCCCATGCACCAGGCCCTGTCAAGTGCAG(+AAAAAA)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606160607_7msb ACAGCCTGTCTCTGCTCCCCATGAATCCTAGG(+AAAAAA)GATCCCCATGCAGGTTCACAAGCTTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606160605_hs4v GCTTGCTACACAAGCTTGTGAACCTGCATGGG(+AAAAAA)GATCCCTAGGATTCATGGGGAGCAGAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606160604_laia TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+AAAAAA)GTACCTGCACTTGACAGGGCCTTAAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606160603_dk6a ACTCCCCATGCTTAAGGCCCTGTCAAGTGCAG(+AAAAAA)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606160601_4g5d CTTGACAGGGCCTTAAGCATGGGGAGTGCAGG(+AAAAAA)GATCCAGGACCCCCATCCCCGGCCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160559_z8y5 CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+AAAAAA)GATCCCTGCACTCCCCATGCTTAAGGCCCTG NGG 10 8 17 8 24 0 100 5 10