Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160526_s441 AGGGGAAATACATTGAGCCCTGCCCACCCATG(+AAAAAA)GATCCCGAATCTGCAGATTCAACAAACTATA NGG 10 8 17 8 24 0 100 5 10
    240606160524_wijn CCACCTATAGTTTGTTGAATCTGCAGATTCGG(+AAAAAA)GATCCATGGGTGGGCAGGGCTCAATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606160523_k97n TCCTGGAGTGTGAGTTGTGTTGAACCTCAGTG(+AAAAAA)GATCCAACAACTTAAAAGCTTTACCACACTG NGG 10 8 17 8 24 0 100 5 10
    240606160522_p1pg ATCAGCAGTGTGGTAAAGCTTTTAAGTTGTTG(+AAAAAA)GATCCACTGAGGTTCAACACAACTCACACTC NGG 10 8 17 8 24 0 100 5 10
    240606160521_npu3 CCTGGTGTATGGGGTCGGGGATAGGGGTCTTG(+AAAAAA)GATCCCTGCACTCCCCATGCTCCAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160519_845g CTTGACAGGGTCTGGAGCATGGGGAGTGCAGG(+AAAAAA)GATCCAAGACCCCTATCCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160518_hf9o CCTGGTGTATGGGGTCGGGGATAGGGGTCTTG(+AAAAAA)GATCCCTGCACTCCCCATGCTCCAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160516_3qwl CTTGACAGGGTCTGGAGCATGGGGAGTGCAGG(+AAAAAA)GATCCAAGACCCCTATCCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160515_weqw GACTCCAGGACATGAAATGCTGTTCTCACTTG(+AAAAAA)GTACCACGTTTTCTACACAGAACCACATCCT NGG 10 8 17 8 24 0 100 5 10
    240606160513_6n95 TGGTAAGGATGTGGTTCTGTGTAGAAAACGTG(+AAAAAA)GTACCAAGTGAGAACAGCATTTCATGTCCTG NGG 10 8 17 8 24 0 100 5 10