Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160442_fm1x GGATGAGAAGTCTGAACTCTACTGCACAACTG(+AAAAAA)GATCCCTAGATTGAGCAGGCTTTATCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606160440_23ej GCTTTTGAAAGATAAAGCCTGCTCAATCTAGG(+AAAAAA)GATCCAGTTGTGCAGTAGAGTTCAGACTTCT NGG 10 8 17 8 24 0 100 5 10
    240606160439_yr8s CTGATGCCCATAGAGATCAGAAGAGGGCACTG(+AAAAAA)GATCCCATGCATCGGGAGTATTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606160437_wg2j GCCCACAACTATCCAATACTCCCGATGCATGG(+AAAAAA)GATCCAGTGCCCTCTTCTGATCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240606160436_ncw1 TCAACTTCCGGGAGGAAGGATGACTAGAGATG(+AAAAAA)GTACCAAAGGTGGGAGACGCTTGGGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240606160434_hahp TGGCAAGGGTGCCCAAGCGTCTCCCACCTTTG(+AAAAAA)GTACCATCTCTAGTCATCCTTCCTCCCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606160432_suf2 AAATAAATCAGAGGAACCCTGGACCCAGCCAG(+AAAAAA)GATCCTACCCTTTGGGGGATTGGAGGCGTGC NGG 10 8 17 8 24 0 100 5 10
    240606160431_0yo6 AGGCTGCACGCCTCCAATCCCCCAAAGGGTAG(+AAAAAA)GATCCTGGCTGGGTCCAGGGTTCCTCTGATT NGG 10 8 17 8 24 0 100 5 10
    240606160429_h6u7 CTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAG(+AAAAAA)GATCCTCCAGCCGCAATAGGCCATCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606160427_nx67 CTTTGGACCTAGATGGCCTATTGCGGCTGGAG(+AAAAAA)GATCCTGAGGACAGGAGAGGGGCTTCGGCTG NGG 10 8 17 8 24 0 100 5 10