Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160542_v86p CCGATACACCAGGTCATTCCTCTGCCCACTTG(+AAAAAA)GTACCTGGTGTACTAAAGGACTGCATGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606160541_yqdr CTGCAAAGACATGCAGTCCTTTAGTACACCAG(+AAAAAA)GTACCAAGTGGGCAGAGGAATGACCTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606160539_l01n CTTGACAGGGCCTGGCGCATGGGGAGTGCAGG(+AAAAAA)GATCCAGGACCCCCATCCCCAACCCGATACA NGG 10 8 17 8 24 0 100 5 10
    240606160537_qait CCTGGTGTATCGGGTTGGGGATGGGGGTCCTG(+AAAAAA)GATCCCTGCACTCCCCATGCGCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160536_1171 ACTCCCCATGCTCCAGGCCCTGTCAAGTGCAG(+AAAAAA)GTACCTGCACTAACTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606160534_k4k8 TCAGCTGCAGTGAAATGCTGGAGTTAGTGCAG(+AAAAAA)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606160533_duwu CCTGGTGTATGGGGTCGGGAATGGGGGTCCTG(+AAAAAA)GATCCCTGCACTCCCCATGCTCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160531_zgoz CTTGACAGGGCCTGGAGCATGGGGAGTGCAGG(+AAAAAA)GATCCAGGACCCCCATTCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160530_7cvn ACTCCCCATGCTCCAGGCCCTGTCAAGTGCAG(+AAAAAA)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606160528_1qa4 TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+AAAAAA)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10