Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160511_rpe3 TATGCAGAATCCTTGGGAAGCTAGAATATGGG(+AAAAAA)GTACCATCCTCATAGCCCCAGAGAGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606160510_kzjv GGCTTCTCCCACTCTCTGGGGCTATGAGGATG(+AAAAAA)GTACCCCATATTCTAGCTTCCCAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240606160508_apmx AGTGAGCCATCTGTGAGATCTCCATAGTCTAG(+AAAAAA)GATCCACTGGTCCCAGGGGGACTGAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606160506_pmqq CTATCAGTCACTCAGTCCCCCTGGGACCAGTG(+AAAAAA)GATCCTAGACTATGGAGATCTCACAGATGGC NGG 10 8 17 8 24 0 100 5 10
    240606160505_e4op GAGGGTCCAGCCACCACTGTCTTCTTTCTCTG(+AAAAAA)GATCCAGAACAAGGAAATCTAGATGAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240606160504_ougi GCCACCCGGCTCATCTAGATTTCCTTGTTCTG(+AAAAAA)GATCCAGAGAAAGAAGACAGTGGTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606160503_l575 GTGTGCAGTTCAATCCCGAGGACTGGAATTTG(+AAAAAA)GATCCCACCACACACATAACAAGCCACATAT NGG 10 8 17 8 24 0 100 5 10
    240606160501_d376 ACAGAATATGTGGCTTGTTATGTGTGTGGTGG(+AAAAAA)GATCCAAATTCCAGTCCTCGGGATTGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606160459_lbbm GCCCCAAGGTCACGTGTTAGGATACTATATGG(+AAAAAA)GTACCATACATTTCAAAGTTGCTCATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606160458_ir77 GCTCATTTCCATGAGCAACTTTGAAATGTATG(+AAAAAA)GTACCCATATAGTATCCTAACACGTGACCTT NGG 10 8 17 8 24 0 100 5 10