Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155731_plm0 TTCTCTCCCGGTAGGCTGCTCTTCCGCGGTGG(+AAAAAA)GATCCAGGTTAGCTGTTTGTAGGATTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606155728_6nol GTCTGGGAAAAGGGGTCCTTTGGTTTCCCTGG(+AAAAAA)GTACCTTGTTCCAGACAGTCTTATTTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606155726_63s8 CTAGGAAGGCAAATAAGACTGTCTGGAACAAG(+AAAAAA)GTACCCAGGGAAACCAAAGGACCCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606155725_b0o9 AGAGGGGGCGGGGTTCCTGGTGGGAAAGCTGG(+AAAAAA)GTACCTGCTTCCTGTTTCCAGCCAGTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606155723_d1h1 AGCAAAGGCAACTGGCTGGAAACAGGAAGCAG(+AAAAAA)GTACCCAGCTTTCCCACCAGGAACCCCGCCC NGG 10 8 17 8 24 0 100 5 10
    240606155722_704v GGGAGGAGGCGCTAGGGAGGCCTGAGTAGTGG(+AAAAAA)GTACCCTCGGAACAGGGTTCCCAGGACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606155719_vhbe AACTCCACAGTCCTGGGAACCCTGTTCCGAGG(+AAAAAA)GTACCCACTACTCAGGCCTCCCTAGCGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606155718_pn16 GGTTTTTATTTGCCTAATCTCTCCCCACATAG(+AAAAAA)GTACCAAGGAAGGGTGACTTCAGAGGGTACA NGG 10 8 17 8 24 0 100 5 10
    240606155716_wbf4 CCCTGTGTACCCTCTGAAGTCACCCTTCCTTG(+AAAAAA)GTACCTATGTGGGGAGAGATTAGGCAAATAA NGG 10 8 17 8 24 0 100 5 10
    240606155714_jo9u CACACACCTGCTTCCTGGGTCCTATGGACAGG(+AAAAAA)GTACCCACCATACCCTTCCTATCCCTCCCTC NGG 10 8 17 8 24 0 100 5 10