Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155621_aya0 CGTTTTTCTTTGCACAGCAGGACTTTAGAAGG(+AAAAAA)GATCCAAAAACCTACCCCAAACTAGAATCTC NGG 10 8 17 8 24 0 100 5 10
    240606155619_94dd GTAATGCCAGCACTAGGCAGACAGGGACCTAG(+AAAAAA)GATCCTGGGGCCAAGGTGTTAGACTAGACAA NGG 10 8 17 8 24 0 100 5 10
    240606155616_s2bi CCAGCTTGTCTAGTCTAACACCTTGGCCCCAG(+AAAAAA)GATCCTAGGTCCCTGTCTGCCTAGTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606155614_5c3r AGGTGTGACCAAGAACTCCTGGACTGAAGAGG(+AAAAAA)GATCCCTTGCATCCTGAGGGCTCTGTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606155612_a2iq ACATCGGGGCACAGAGCCCTCAGGATGCAAGG(+AAAAAA)GATCCCTCTTCAGTCCAGGAGTTCTTGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606155611_aorh CTTCTGTCCCAGATTCTGAGTCCTTTGCAGAG(+AAAAAA)GATCCTGAGAATAATGTCACCTTGAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606155610_2x72 ACTCACTCTCCTCAAGGTGACATTATTCTCAG(+AAAAAA)GATCCTCTGCAAAGGACTCAGAATCTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606155608_kct9 TCATTATTGACAGCCGGACACCCATGGCCCAG(+AAAAAA)GATCCCTCCACCCTTGGTCACTCTGACCTCT NGG 10 8 17 8 24 0 100 5 10
    240606155606_y6sl GAGCCAGAGGTCAGAGTGACCAAGGGTGGAGG(+AAAAAA)GATCCTGGGCCATGGGTGTCCGGCTGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606155604_rlvv GGAGGGCTCATCGACAAGTAGCCCCCAGCTGG(+AAAAAA)GATCCGCAACATCCACCTCCATCTCCTCAGA NGG 10 8 17 8 24 0 100 5 10