Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155603_1pt6 ACGGATCTGAGGAGATGGAGGTGGATGTTGCG(+AAAAAA)GATCCCAGCTGGGGGCTACTTGTCGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240606155601_14g1 TCCTCCTTCCCCTGGCTAGCACTCTGGTCAGG(+AAAAAA)GTACCCCTTTATTCTGCTGCAGAGATCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606155559_6ypg TGTGTTTAAGATCTCTGCAGCAGAATAAAGGG(+AAAAAA)GTACCCTGACCAGAGTGCTAGCCAGGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606155557_npu5 CCATTCCCCCATTCGACATGGGATTTCACCAG(+AAAAAA)GATCCACCTATATCCCCCTTTTTTTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606155555_vjho AAATATCTTTCAAAAAAAGGGGGATATAGGTG(+AAAAAA)GATCCTGGTGAAATCCCATGTCGAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240606155554_kkia GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+AAAAAA)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606155553_5aj5 CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+AAAAAA)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240606155551_q19p GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+AAAAAA)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606155549_wgvb CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+AAAAAA)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240606155548_9u7b TGCCAGCTCAGGGTCCCCTCCACTGGTTCTTG(+AAAAAA)GTACCTACCACCCTCCTTTAAAGACCCAGAG NGG 10 8 17 8 24 0 100 5 10