Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155547_3bz1 GGTGCCTCTGGGTCTTTAAAGGAGGGTGGTAG(+AAAAAA)GTACCAAGAACCAGTGGAGGGGACCCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606155545_vgd0 TGTCTGTTCCTGCTTTTCTGTCCTGGGCTGGG(+AAAAAA)GATCCAGGCTCAGCACTTTCTCCCCGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606155543_gahj ATAGTGGGGACGGGGAGAAAGTGCTGAGCCTG(+AAAAAA)GATCCCCAGCCCAGGACAGAAAAGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606155542_3bx3 TTAAGAAATTAATATAGAAGGAGATGAAAGTG(+AAAAAA)GATCCTGAAAGGGTGACAGGAGGAATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606155541_6v9g TAACATTTCCATTCCTCCTGTCACCCTTTCAG(+AAAAAA)GATCCACTTTCATCTCCTTCTATATTAATTT NGG 10 8 17 8 24 0 100 5 10
    240606155539_enqh AACCTGTTCCTGGAGGTGGAACTCTTCATCAG(+AAAAAA)GATCCCCAAAGGCGTGCTGGAATTAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606155537_2eop GTTCAGTGGACTAATTCCAGCACGCCTTTGGG(+AAAAAA)GATCCTGATGAAGAGTTCCACCTCCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606155536_bfpl TTCTATAACCAATACCTTGACCCTCTACAGTG(+AAAAAA)GATCCAGAAATGCTGAGAACCCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606155535_2z6y GGTGGTCAGCCTGTGGGTTCTCAGCATTTCTG(+AAAAAA)GATCCACTGTAGAGGGTCAAGGTATTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606155533_cjno AATAGGCCAGACCATTCTACACTTTTCCACTG(+AAAAAA)GATCCTTTCCTTATTTTTTTTGGATCATAAT NGG 10 8 17 8 24 0 100 5 10