Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153730_6nn0 TCAGACGTTGTTTTGACCAAAGTGTTCACTGG(+AAAAAA)GATCCTCCAGGAAGTACCAGGGAACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606153729_2gy6 GTTATGCTGCAGTTCCCTGGTACTTCCTGGAG(+AAAAAA)GATCCCAGTGAACACTTTGGTCAAAACAACG NGG 10 8 17 8 24 0 100 5 10
    240606153728_v59u CACATTAGTTAGTAACCACAGAACTGGTTTTG(+AAAAAA)GATCCTGGAATTAAAGCATTAATATCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606153726_clfy ATTTACCTTTGATATTAATGCTTTAATTCCAG(+AAAAAA)GATCCAAAACCAGTTCTGTGGTTACTAACTA NGG 10 8 17 8 24 0 100 5 10
    240606153725_gwjd GTGTCAGTCTCCAAGCTTACCTTTTATACAAG(+AAAAAA)GATCCCAGTCTTGGGTTGGGGCTCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606153723_1n3a AGTAGACAGGGTGAGCCCCAACCCAAGACTGG(+AAAAAA)GATCCTTGTATAAAAGGTAAGCTTGGAGACT NGG 10 8 17 8 24 0 100 5 10
    240606153721_qh6x GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+AAAAAA)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606153719_bw15 CCTGGTGAAAACAGTTGATGCTGAATATTGGG(+AAAAAA)GATCCTGGTAGCCATGCTCCTGGAGCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606153718_pgjk CTGATACCTTCTTCAGCTCTTATTTAAGGAAG(+AAAAAA)GTACCGTTTATGTTTCAAAACTTAGATATTC NGG 10 8 17 8 24 0 100 5 10
    240606153717_6rpw GCCCAGAATATCTAAGTTTTGAAACATAAACG(+AAAAAA)GTACCTTCCTTAAATAAGAGCTGAAGAAGGT NGG 10 8 17 8 24 0 100 5 10