Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153835_dlqc CATGAAAGAGTTATTAGCCACCAAGTATGCTG(+AAAAAA)GTACCTATAATCCCAGAACACAGAAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606153834_ar74 CTGCCTCAGCCTTCTGTGTTCTGGGATTATAG(+AAAAAA)GTACCAGCATACTTGGTGGCTAATAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240606153832_e603 GCATGAAGTTATCTGTGAGATGAGGGTAGGAG(+AAAAAA)GATCCTGGGAGTCAGGACCACCCAGAACCAA NGG 10 8 17 8 24 0 100 5 10
    240606153830_46cj GTGGGTTGGTTCTGGGTGGTCCTGACTCCCAG(+AAAAAA)GATCCTCCTACCCTCATCTCACAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240606153827_8q8q CCTGGAGGGCCTGGGCTGGAAATTTGCCCCTG(+AAAAAA)GATCCAGAACCCCATTCGTTTTACAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606153826_85yh CCTATCCCAACTGTAAAACGAATGGGGTTCTG(+AAAAAA)GATCCAGGGGCAAATTTCCAGCCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606153825_36ja TGCGTTCTTGCCATGAGCGAGCTGACTATCAG(+AAAAAA)GTACCTGTTCTCGCTCTGTCTGGGAACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606153823_rn0p TGTGACACAGTTCCCAGACAGAGCGAGAACAG(+AAAAAA)GTACCTGATAGTCAGCTCGCTCATGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606153821_5pwq GGCTAAGGATGGCCCTAAACATCCATCCTTCG(+AAAAAA)GATCCTCCTGCCTCCTTTTCTCTAGTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240606153820_1a37 TGATCCCCGCACTAGAGAAAAGGAGGCAGGAG(+AAAAAA)GATCCGAAGGATGGATGTTTAGGGCCATCCT NGG 10 8 17 8 24 0 100 5 10