Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153922_bmh6 GTGTTAAAGAGTCCTGCATGAGCTGCACTGAG(+AAAAAA)GATCCCTAAGTACAATATATCTGATTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606153920_8w5t CAGGGAGGCTAATCAGATATATTGTACTTAGG(+AAAAAA)GATCCTCAGTGCAGCTCATGCAGGACTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606153919_79lc CACCCAACTAAGGGGCATGGATGCCCAGTGTG(+AAAAAA)GATCCTGTGGGGATTCTGTTGTCTCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606153917_mb2q ATGAAGGAAAGGAGACAACAGAATCCCCACAG(+AAAAAA)GATCCACACTGGGCATCCATGCCCCTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606153915_7s51 TGCAGGGCAGTGGGAGATGGTCTCATTTGGGG(+AAAAAA)GTACCCTTTAGTGAACCTGTCTTCCCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606153913_ssh8 ATACTCTAAGGGGAAGACAGGTTCACTAAAGG(+AAAAAA)GTACCCCCAAATGAGACCATCTCCCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606153911_ybqn GAGCATCCCGCCTTGCTCTCCTCCCCTCCCTG(+AAAAAA)GATCCCCCCATTAAAGCTGGAGGTGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606153908_2iky CAAAGGCAGCCCACCTCCAGCTTTAATGGGGG(+AAAAAA)GATCCAGGGAGGGGAGGAGAGCAAGGCGGGA NGG 10 8 17 8 24 0 100 5 10
    240606153907_eqxo GTCTGCAGCTCCATTACTTTTCTCAGTCAGTG(+AAAAAA)GATCCAAGGGACCAAGATCCATTGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240606153905_8bck ATTTGCATCTGCCAATGGATCTTGGTCCCTTG(+AAAAAA)GATCCACTGACTGAGAAAAGTAATGGAGCTG NGG 10 8 17 8 24 0 100 5 10