Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155250_nico AGTTGTATTAATTTTCCCTAGCAGACATATTG(+AAAAAA)GTACCTGCACGCTTGCTGCTAGAGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606155249_h7ch ATTAAAAGTGGGTAAGCTGCTGGCTGCAGATG(+AAAAAA)GTACCAGCTAAAGAGCATTGTACCTAAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606155248_6j6q TTGCCACCCTTAGGTACAATGCTCTTTAGCTG(+AAAAAA)GTACCATCTGCAGCCAGCAGCTTACCCACTT NGG 10 8 17 8 24 0 100 5 10
    240606155246_txbu TAGAATGCACGTTTTTCATAAACACCCAGGTG(+AAAAAA)GTACCCACAGCTACCATTACTCAGGGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606155245_hzhw TGTAGAAGGCCCCTGAGTAATGGTAGCTGTGG(+AAAAAA)GTACCACCTGGGTGTTTATGAAAAACGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606155243_70ht ATTGCCCCCAGACGAGTCATTGCAGGCTCCTG(+AAAAAA)GATCCTAACAAAAAGAGCAGGGCTTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606155242_5g2u TGGTCCACAGGAAGCCCTGCTCTTTTTGTTAG(+AAAAAA)GATCCAGGAGCCTGCAATGACTCGTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606155240_uvge CCATGAGTACTGGAGATACAGGCATAAGACAG(+AAAAAA)GATCCATGCCTTCTTTCTACCATGGGAAACC NGG 10 8 17 8 24 0 100 5 10
    240606155239_190o CCCTGGGTTTCCCATGGTAGAAAGAAGGCATG(+AAAAAA)GATCCTGTCTTATGCCTGTATCTCCAGTACT NGG 10 8 17 8 24 0 100 5 10
    240606155238_u6st GGGTGCTCCTTATGGGATCTTCTACCCAGTGG(+AAAAAA)GATCCTCCAGGTTCCTCTGTCCTGTCATCCC NGG 10 8 17 8 24 0 100 5 10