Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154024_ym77 AGGCTCTCTGTAGGTCTTGGATATCAAACCTG(+AAAAAA)GATCCTTTGGAAAACTAACAAGTGCTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606154022_skgv GAGCCCGCGCGCCCCGCTGCCCTCCTCGCCCG(+AAAAAA)GATCCCTCTCCTGCCCTCTGACTGGAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606154020_dssq GTGTAGAGACTCCAGTCAGAGGGCAGGAGAGG(+AAAAAA)GATCCGGGCGAGGAGGGCAGCGGGGCGCGCG NGG 10 8 17 8 24 0 100 5 10
    240606154019_s8ww CACCCTGGAGCAGCTAGGGGTTCACGGACCTG(+AAAAAA)GATCCTACACAGATCCCAGGTTCCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606154017_ecgk GATGGGAGGAGGGGAACCTGGGATCTGTGTAG(+AAAAAA)GATCCAGGTCCGTGAACCCCTAGCTGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606154016_c4h7 CTCTGGGCTCAACAGCCCTGGAGAAGGACGAG(+AAAAAA)GATCCCCATCCTCCAGCAAGTCCCAACACAT NGG 10 8 17 8 24 0 100 5 10
    240606154014_rgaq CCAATATGTGTTGGGACTTGCTGGAGGATGGG(+AAAAAA)GATCCTCGTCCTTCTCCAGGGCTGTTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606154013_w5m5 GGGGCACACACACGGACACACACACACACACG(+AAAAAA)GATCCAAGGGAGGGGGCTGAAAGATATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606154010_bvhj CTCTCAGCCATATCTTTCAGCCCCCTCCCTTG(+AAAAAA)GATCCGTGTGTGTGTGTGTGTCCGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606154008_h7qy AGCCCCTGGCACTTGAGGTGTGTGACCATTGG(+AAAAAA)GATCCGGATAGCCGCAGCGGGGCTGAGGTCT NGG 10 8 17 8 24 0 100 5 10