Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155304_ys4x ATCTAGGGGTGGAGGGATGATTGATGGCTGGG(+AAAAAA)GATCCTACAGGTCTTGGGACCTGGGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606155302_30i5 CCCGGGCCTGGCGTGATTAAAGATGAAGCCTG(+AAAAAA)GATCCATCTTCACCAAAGCTGAAAACCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606155301_i5he GCTGCGCCTGGTTTTCAGCTTTGGTGAAGATG(+AAAAAA)GATCCAGGCTTCATCTTTAATCACGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606155300_f15j CAGCCAAAATGCACCAGACGCAGAAGCTAGTG(+AAAAAA)GATCCTCAGGGCTCCATACAACCCCTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240606155258_ovxc GCAGTACTAGAGGGGTTGTATGGAGCCCTGAG(+AAAAAA)GATCCACTAGCTTCTGCGTCTGGTGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240606155257_5nn7 TTCATGTTGTTGGGAAAACTTAGCAGAAATCG(+AAAAAA)GTACCTCGGTCATTACAAAGAAGCACGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606155255_bf0f TCCTTTGAACGTGCTTCTTTGTAATGACCGAG(+AAAAAA)GTACCGATTTCTGCTAAGTTTTCCCAACAAC NGG 10 8 17 8 24 0 100 5 10
    240606155254_c2oa TGCAGGGAAAATACGAGGGGACTCCACTAATG(+AAAAAA)GTACCTTCATAAAACCACTCCCTGCTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606155253_np1t GGCAAGAAAAAGCAGGGAGTGGTTTTATGAAG(+AAAAAA)GTACCATTAGTGGAGTCCCCTCGTATTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606155251_1uso CGCCCACCAAGGCTCTAGCAGCAAGCGTGCAG(+AAAAAA)GTACCAATATGTCTGCTAGGGAAAATTAATA NGG 10 8 17 8 24 0 100 5 10