Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154040_zbq9 CGGGCGAACCCCGTGTGCTGGGTCTGGCCATG(+AAAAAA)GTACCAGGACACCACATCGTTTCTATTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606154038_swag CCCAAGCCGGTTCCTTACCGCGGCCTGAGCCG(+AAAAAA)GATCCTACCATCCCTTTGATGGAACAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606154037_0op1 CACATTCAGCTGTTCCATCAAAGGGATGGTAG(+AAAAAA)GATCCGGCTCAGGCCGCGGTAAGGAACCGGC NGG 10 8 17 8 24 0 100 5 10
    240606154035_dnss TCAAGACATTGTAGTGAGGGACCCACAAATGG(+AAAAAA)GTACCTTTTTTTTTCCAGTAGGCCTTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606154033_o7r9 TGTTAGTGGCAAGGCCTACTGGAAAAAAAAAG(+AAAAAA)GTACCCATTTGTGGGTCCCTCACTACAATGT NGG 10 8 17 8 24 0 100 5 10
    240606154032_0vsv CCCATGCAACCTAAGAGTCCATAAATTGTTTG(+AAAAAA)GATCCATCAATCGTGATATTTAGTAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606154031_wene ATTTATGAGCTTACTAAATATCACGATTGATG(+AAAAAA)GATCCAAACAATTTATGGACTCTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606154030_1zku ACATTGTCAACCACAGACCACTGCTAAAATTG(+AAAAAA)GATCCTTTGCTTACACCACAGACTCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240606154028_ib4z GCGTTTGTTCAGAGTCTGTGGTGTAAGCAAAG(+AAAAAA)GATCCAATTTTAGCAGTGGTCTGTGGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240606154026_re94 AGTGGTGAAGAGCACTTGTTAGTTTTCCAAAG(+AAAAAA)GATCCAGGTTTGATATCCAAGACCTACAGAG NGG 10 8 17 8 24 0 100 5 10