Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154129_eiml CTGTGGGTGGGGACTGGGGTTTCGGGCCACTG(+AAAAAA)GATCCTCCTCAGACTTGTCCCCCCCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240606154128_yvyg CCCTAGGTTGTTCCCAGGGCTGCAAAGCGTAG(+AAAAAA)GTACCAACACTTTCTTGTTCCCCTCCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606154127_t0o2 CACCCTTCCTGGAGGGGAACAAGAAAGTGTTG(+AAAAAA)GTACCTACGCTTTGCAGCCCTGGGAACAACC NGG 10 8 17 8 24 0 100 5 10
    240606154125_naet GAGGGAGGTGATGCCTCGGAACAATAAGGATG(+AAAAAA)GATCCCTTCCTTCAACCCATGCTAGGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606154123_ufa7 TTCCCTCTCCCCTAGCATGGGTTGAAGGAAGG(+AAAAAA)GATCCATCCTTATTGTTCCGAGGCATCACCT NGG 10 8 17 8 24 0 100 5 10
    240606154122_5l3c GGGGGCTAGAGCTCCCTTCTGGAATTCCAGAG(+AAAAAA)GATCCTGTATGGAAGTGGGGCTGGACCATGG NGG 10 8 17 8 24 0 100 5 10
    240606154120_2w4o GGACACCATGGTCCAGCCCCACTTCCATACAG(+AAAAAA)GATCCTCTGGAATTCCAGAAGGGAGCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606154119_eord CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+AAAAAA)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606154117_3d6j TAGTTTAGCTTCCGATTGTGGCACTCTGAGGG(+AAAAAA)GATCCTTGCCTCCTCACTAATAGCTGTAGCG NGG 10 8 17 8 24 0 100 5 10
    240606154116_zmsc CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+AAAAAA)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10