Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154055_041s AATAAAAATAGTATCTTTTCAAAATCCACAGG(+AAAAAA)GATCCAGGGAGGAGCTTGTCCCCAGCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606154054_z5gt AAAATGCCACGGTCCTGCTCTGGTCAATAAAG(+AAAAAA)GATCCTTTGAAGATATGCAAGTGGTGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606154053_3qrq TAAGGAAGACCACCACTTGCATATCTTCAAAG(+AAAAAA)GATCCTTTATTGACCAGAGCAGGACCGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606154052_74or TAATAGGGGTGGGGAATCAATAAACATGAATG(+AAAAAA)GTACCTACTACTCCTTTCTTTCCTCATTAAG NGG 10 8 17 8 24 0 100 5 10
    240606154051_21zn GTACTCTTAATGAGGAAAGAAAGGAGTAGTAG(+AAAAAA)GTACCATTCATGTTTATTGATTCCCCACCCC NGG 10 8 17 8 24 0 100 5 10
    240606154048_po06 TAATCCCACTACTTGGGAGGCAGAGGCAGGGG(+AAAAAA)GATCCCTTGAGTTCAAGGCCAGTCTGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606154047_2i8u TTAGTTAGACCAGACTGGCCTTGAACTCAAGG(+AAAAAA)GATCCCCCTGCCTCTGCCTCCCAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606154045_809y CTCCTGGCTATAGCCCTCATCTCCAATTTTAG(+AAAAAA)GATCCCTTAAGACTTGACTACACCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606154044_70zg AGGTCAGGAAAAGGTGTAGTCAAGTCTTAAGG(+AAAAAA)GATCCTAAAATTGGAGATGAGGGCTATAGCC NGG 10 8 17 8 24 0 100 5 10
    240606154042_ygew TTGGACTTCAATAGAAACGATGTGGTGTCCTG(+AAAAAA)GTACCATGGCCAGACCCAGCACACGGGGTTC NGG 10 8 17 8 24 0 100 5 10