Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154145_j7f2 CAGCCGCGAGCCGTTGGGCCCTATCGTGCCTG(+AAAAAA)GATCCTTGCCCCAAACCCTGAGTTCTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606154144_yvzd TCCAGCAAACATTTATTGATAACCCAAGTAAG(+AAAAAA)GTACCCTGCAGATGTAGAAAAAAGCATCATG NGG 10 8 17 8 24 0 100 5 10
    240606154142_l72c CCATCCATGATGCTTTTTTCTACATCTGCAGG(+AAAAAA)GTACCTTACTTGGGTTATCAATAAATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606154141_pd2p CTGTAAATATAATTTATTGAGGCCTGTAGTTG(+AAAAAA)GTACCAGGGCCTTTATTCTGTTTGCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240606154138_jvo7 AAGTCGGAAATGCAAACAGAATAAAGGCCCTG(+AAAAAA)GTACCAACTACAGGCCTCAATAAATTATATT NGG 10 8 17 8 24 0 100 5 10
    240606154137_ko1h TGCTTGTTGGTTTCTCTCCAGTTCTAGAACTG(+AAAAAA)GATCCTTTAGTTTTCCTGGGCTTAGCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606154136_dh2m TATTCTCAGGGCTAAGCCCAGGAAAACTAAAG(+AAAAAA)GATCCAGTTCTAGAACTGGAGAGAAACCAAC NGG 10 8 17 8 24 0 100 5 10
    240606154134_7bs7 CTAAGTGGTTCACAACCACCTAGAATCCAGAG(+AAAAAA)GATCCGATATCCTCTTCTGGTATCCGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606154133_hzd6 GGGGTGCCCACGGATACCAGAAGAGGATATCG(+AAAAAA)GATCCTCTGGATTCTAGGTGGTTGTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240606154131_an77 AGGAAAAGTAAGGGGGGGACAAGTCTGAGGAG(+AAAAAA)GATCCAGTGGCCCGAAACCCCAGTCCCCACC NGG 10 8 17 8 24 0 100 5 10