Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153612_li24 CTCTCTCTTCCAGGAGAGGTGGGTGGTCTTGG(+AAAAAA)GATCCGCCCCTCCCCCGCCCCCAGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606153611_5rw6 TGAGACAGGGTTCTGGGGGCGGGGGAGGGGCG(+AAAAAA)GATCCCAAGACCACCCACCTCTCCTGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606153609_cjq9 AAATAAATATATATGTTAAATAAAAGGCCCAG(+AAAAAA)GTACCAAAGAGCAAAATTTAATGAAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606153607_ge9m CAGTTCTGGCTTTCATTAAATTTTGCTCTTTG(+AAAAAA)GTACCTGGGCCTTTTATTTAACATATATATT NGG 10 8 17 8 24 0 100 5 10
    240606153606_8de0 AGGCTCCACAGAGAGTCCTACTCCACCATGGG(+AAAAAA)GATCCAGTATAGACATCAAGCAAAGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606153604_p04c CTTGCCTTTTCCTTTGCTTGATGTCTATACTG(+AAAAAA)GATCCCCATGGTGGAGTAGGACTCTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606153603_fgph GTATGCGTTCATGGTGGAACACAACCACATCG(+AAAAAA)GTACCTACTTCAGAACAATTATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606153602_q8cj GTTTTTTTTCTCAGATAATTGTTCTGAAGTAG(+AAAAAA)GTACCGATGTGGTTGTGTTCCACCATGAACG NGG 10 8 17 8 24 0 100 5 10
    240606153600_r217 AGCTTCAGGACTTGTTCCCCCTCTGCCGGTGG(+AAAAAA)GATCCTGCCTGAAACCTTCTGCGCAGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606153559_l5b8 CTGAATTCTTCTGCGCAGAAGGTTTCAGGCAG(+AAAAAA)GATCCCACCGGCAGAGGGGGAACAAGTCCTG NGG 10 8 17 8 24 0 100 5 10