Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153557_ps6m GCAAGAGGTCAAAAGATGTGGGCCATACCAAG(+AAAAAA)GTACCAAGGAAGCTGCCCTCAGCCAAATGCT NGG 10 8 17 8 24 0 100 5 10
    240606153555_mqof TCTGTAGCATTTGGCTGAGGGCAGCTTCCTTG(+AAAAAA)GTACCTTGGTATGGCCCACATCTTTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606153554_w1yq TGCCTCCCTAGGTCTTCGGCTTGGCCCTACAG(+AAAAAA)GTACCTGTGCTTTGCCAAGGGTTACTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240606153553_gmv2 GGCATGCTCCAGTAACCCTTGGCAAAGCACAG(+AAAAAA)GTACCTGTAGGGCCAAGCCGAAGACCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606153552_2sfp GCCGTGCCTGCAGAGGTCAGGAGAGGGAATTG(+AAAAAA)GATCCCCTGGGACTGGAGTTATAGATGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606153549_w3kz ACTCATGACCATCTATAACTCCAGTCCCAGGG(+AAAAAA)GATCCAATTCCCTCTCCTGACCTCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606153546_oblh GGTAACTCGAAGCTGTCACTCCATTTCCAGGG(+AAAAAA)GATCCTGTGCACTCTACTGGCCTCTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606153545_96nc GCAGTGCCCTTAGAGGCCAGTAGAGTGCACAG(+AAAAAA)GATCCCCTGGAAATGGAGTGACAGCTTCGAG NGG 10 8 17 8 24 0 100 5 10
    240606153544_t044 TGTTCCTCTTCCCTGCTGAGTATAGGTAGCTG(+AAAAAA)GATCCTGCGGTCCACTTTGCTTACTATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606153542_cq0z GGCAGTTAGATAGTAAGCAAAGTGGACCGCAG(+AAAAAA)GATCCAGCTACCTATACTCAGCAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10