Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145940_lip3 TGGTCTTTATTTAGAGTCCATAACCCCTCCAG(+AAAAAA)GTACCTCAATCCTTCCCATAAATCTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606145939_68m8 GATCTTGTGGAAGATTTATGGGAAGGATTGAG(+AAAAAA)GTACCTGGAGGGGTTATGGACTCTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606145938_nj63 GGTTAGACAGGCCAGCCCAGCTAGGAGAGCAG(+AAAAAA)GATCCACAGGTAGACGGCAGAGTCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606145936_013o GTGGCTTACCATGACTCTGCCGTCTACCTGTG(+AAAAAA)GATCCTGCTCTCCTAGCTGGGCTGGCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606145935_0fzl GAAACAGCCAAAGAATGGATAAAGAAAATGAG(+AAAAAA)GTACCTTTACATAATGGAATATTACTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606145934_0m56 TTAATATTTGAGTAATATTCCATTATGTAAAG(+AAAAAA)GTACCTCATTTTCTTTATCCATTCTTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606145932_y5b3 ATAATAGGGTAATGCTTAGTAGAAGGCACGTG(+AAAAAA)GATCCATACACTAGACTAACTCGGGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606145931_i0kb AATTTTCCACTCCCGAGTTAGTCTAGTGTATG(+AAAAAA)GATCCACGTGCCTTCTACTAAGCATTACCCT NGG 10 8 17 8 24 0 100 5 10
    240606145930_o82g ATTTTATTGCAGTGATACAGATGCCACTGTTG(+AAAAAA)GTACCCTTAAATTTTTATTTCTGCTCACCAA NGG 10 8 17 8 24 0 100 5 10
    240606145928_8z30 TAACCTTGGTGAGCAGAAATAAAAATTTAAGG(+AAAAAA)GTACCAACAGTGGCATCTGTATCACTGCAAT NGG 10 8 17 8 24 0 100 5 10