Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145844_ij62 TAAGAGTCGGCAGCCTGGCAGCGGTACTGGGG(+AAAAAA)GATCCGTGCCCAGGCAGCTGTCAAGCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606145842_3nyh GGGAGGGAATGCTTGACAGCTGCCTGGGCACG(+AAAAAA)GATCCCCCAGTACCGCTGCCAGGCTGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240606145840_o4fc TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+AAAAAA)GATCCCTGCTGCGCATTCTGATGTGAATCCC NGG 10 8 17 8 24 0 100 5 10
    240606145838_l54t ACCTTGGGATTCACATCAGAATGCGCAGCAGG(+AAAAAA)GATCCAGGAATATCTTGCTAGGATGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240606145837_lpz2 GTGGCTGCGTCACACAGAGATGGAAGCAAGTG(+AAAAAA)GATCCTCTGTATGCTTCTGTCTCCAGACGAG NGG 10 8 17 8 24 0 100 5 10
    240606145835_7ig4 CAAAGCTCGTCTGGAGACAGAAGCATACAGAG(+AAAAAA)GATCCACTTGCTTCCATCTCTGTGTGACGCA NGG 10 8 17 8 24 0 100 5 10
    240606145834_x1qv CATGTGCCCACAGAAGCCAGAAGAGGGCGTCG(+AAAAAA)GATCCCTTGGAACTGGAATGGCTGAGTCATC NGG 10 8 17 8 24 0 100 5 10
    240606145831_osml GGAGAGATGACTCAGCCATTCCAGTTCCAAGG(+AAAAAA)GATCCGACGCCCTCTTCTGGCTTCTGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606145829_y7gx GTATTGAGATATGTAAAGACACCTGCAGACGG(+AAAAAA)GTACCTAGGGAGAAGACACACACATACACAC NGG 10 8 17 8 24 0 100 5 10
    240606145826_29e2 AGAATGTGTGTATGTGTGTGTCTTCTCCCTAG(+AAAAAA)GTACCCGTCTGCAGGTGTCTTTACATATCTC NGG 10 8 17 8 24 0 100 5 10