Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145641_gnoc AGTGACAGATTAACAGGGGTAGCTGCTACTTG(+AAAAAA)GATCCACAGAATCTCTGCAAGTGAGGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240606145640_hb0j CATAGTTAACCCTCACTTGCAGAGATTCTGTG(+AAAAAA)GATCCAAGTAGCAGCTACCCCTGTTAATCTG NGG 10 8 17 8 24 0 100 5 10
    240606145638_r2qs CAGCCCCAGCAAGCGCCCAGGAACAGGGAGAG(+AAAAAA)GATCCATGCATGTGAGCAGCCAGCATCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606145637_5m18 GGTGTCCTTGATGCTGGCTGCTCACATGCATG(+AAAAAA)GATCCTCTCCCTGTTCCTGGGCGCTTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606145636_16xv TTGATATAAAGCACAAACTGGCTTTAAACTCG(+AAAAAA)GATCCTTCGGCCTCAGCCTTTCTAGCACCAA NGG 10 8 17 8 24 0 100 5 10
    240606145634_e94l TCTCCTTGGTGCTAGAAAGGCTGAGGCCGAAG(+AAAAAA)GATCCGAGTTTAAAGCCAGTTTGTGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606145633_cjua TGTGGGTAGAAAATGGTAACACTAAACAATGG(+AAAAAA)GATCCCAGTACTCAACTTTAAAGATTTTATT NGG 10 8 17 8 24 0 100 5 10
    240606145631_xhos GAGAAAATAAAATCTTTAAAGTTGAGTACTGG(+AAAAAA)GATCCCATTGTTTAGTGTTACCATTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606145629_5cwp ACACCCCTGAAACTGACAAGTGAGGGGCCAGG(+AAAAAA)GATCCCCCACTGACTGGATTGTGTCTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606145627_uj1k TGATGTGCCCAGACACAATCCAGTCAGTGGGG(+AAAAAA)GATCCCTGGCCCCTCACTTGTCAGTTTCAGG NGG 10 8 17 8 24 0 100 5 10