Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145542_j1rc GAAGGGCCCAACACTGCTGGATCTTGCAGCTG(+AAAAAA)GATCCCACTGCCCAGTCAAGCCACCCTGACC NGG 10 8 17 8 24 0 100 5 10
    240606145541_fsiy GCACTGGTCAGGGTGGCTTGACTGGGCAGTGG(+AAAAAA)GATCCAGCTGCAAGATCCAGCAGTGTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606145539_508v GCTGCTCCTGAGTCCTTTTACAAGGGCAGATG(+AAAAAA)GATCCTGGGGTAACAGGGTGGTACGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606145536_043z GCCCCAGCCCTCGTACCACCCTGTTACCCCAG(+AAAAAA)GATCCATCTGCCCTTGTAAAAGGACTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606145535_51z8 AACCCCAGCACTTGTGTTACCTCAGGGCCTTG(+AAAAAA)GTACCTGGATACTGCCACAGAATTTCCATGG NGG 10 8 17 8 24 0 100 5 10
    240606145533_zgpy CCGCCCCATGGAAATTCTGTGGCAGTATCCAG(+AAAAAA)GTACCAAGGCCCTGAGGTAACACAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606145531_m7aq TTACCAGCCCTCAACCTCAGTTCTGAGGGTGG(+AAAAAA)GATCCCATCGCTCAGCAAGGCTTCATCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606145529_h642 GGAGGTCAGGATGAAGCCTTGCTGAGCGATGG(+AAAAAA)GATCCCACCCTCAGAACTGAGGTTGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606145527_z9b4 CAGCACGCCTGGCCTGCCCACCTCCACCTTGG(+AAAAAA)GTACCTCGTAGCCTAGGGACGGCAGGACTTT NGG 10 8 17 8 24 0 100 5 10
    240606145526_vcx5 GTGTTAAAGTCCTGCCGTCCCTAGGCTACGAG(+AAAAAA)GTACCCAAGGTGGAGGTGGGCAGGCCAGGCG NGG 10 8 17 8 24 0 100 5 10