Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145505_ca2d AGAGGGCAAGGAGCCTGGAGATGGACACACTG(+AAAAAA)GTACCAGGGGGTTACAAACAGTAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606145503_zna1 GTGGAAACCTCAGGTTATTGTACTTGTATGAG(+AAAAAA)GTACCCTAGGCAATTCGGCCACTTCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606145501_0pfd TCCTGATTTCAGAAGTGGCCGAATTGCCTAGG(+AAAAAA)GTACCTCATACAAGTACAATAACCTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606145500_8pix CCAGTGCCCATAGAAGTCAGAAGAGGGCATTG(+AAAAAA)GATCCTCTGGATCAATGGATTTGGAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606145458_86w6 TCATCCCCAGCTCCAAATCCATTGATCCAGAG(+AAAAAA)GATCCAATGCCCTCTTCTGACTTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240606145456_5qp5 GTTGACTGAGATGGCAGCCAGTGTGCTGAGTG(+AAAAAA)GATCCAAACATCACAATGGTGTGACCACTTG NGG 10 8 17 8 24 0 100 5 10
    240606145455_5f3b CCTTTCAAGTGGTCACACCATTGTGATGTTTG(+AAAAAA)GATCCACTCAGCACACTGGCTGCCATCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606145454_oeel TCATGTGTCTTGGAACCCAGTTACTCTAAGAG(+AAAAAA)GTACCTGGTACAATGTGTATGACTCACGTGC NGG 10 8 17 8 24 0 100 5 10
    240606145452_0p6m AAGAAGCACGTGAGTCATACACATTGTACCAG(+AAAAAA)GTACCTCTTAGAGTAACTGGGTTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240606145451_l5rh CGGGCAGAGACTTGCTGAACTTTTAACTTGAG(+AAAAAA)GATCCTTCTCTTGAGAAAACCTGTGGGCATT NGG 10 8 17 8 24 0 100 5 10