Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145416_pokt CAATCCTCTTTGGTTGGTAATAGACTTTTTTG(+AAAAAA)GATCCATGTTTGATTTGAATCTAGCTACTTA NGG 10 8 17 8 24 0 100 5 10
    240606145414_j2ef ATGCTGTGCCTCTCATCCAAGTATTAACAGAG(+AAAAAA)GTACCTGCCATCTTGGGAACCTTAAAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606145413_41l5 TAGTTATTCCTTTAAGGTTCCCAAGATGGCAG(+AAAAAA)GTACCTCTGTTAATACTTGGATGAGAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606145412_1ahh AACACTTTTAAAAAAATTCATTTATAAATCAG(+AAAAAA)GTACCCATTCCCACAATCTTAGGGTCTAGTC NGG 10 8 17 8 24 0 100 5 10
    240606145410_dtvx ATCCAGACTAGACCCTAAGATTGTGGGAATGG(+AAAAAA)GTACCTGATTTATAAATGAATTTTTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606145409_6v1y CAGGAGAGGTTCCTGGTCTGTGTCAAAGTCTG(+AAAAAA)GTACCATAAAAACCAAGTAAGGATGGATCTG NGG 10 8 17 8 24 0 100 5 10
    240606145408_5dtn CCTGTCAGATCCATCCTTACTTGGTTTTTATG(+AAAAAA)GTACCAGACTTTGACACAGACCAGGAACCTC NGG 10 8 17 8 24 0 100 5 10
    240606145406_trzg CTACAAAGTCTCTCCTGTGCCTTCCCAGATAG(+AAAAAA)GTACCTAGGCTGCATCCTGGAGCCACGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606145404_znrr GCTATGGAACGTGGCTCCAGGATGCAGCCTAG(+AAAAAA)GTACCTATCTGGGAAGGCACAGGAGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606145403_lgxi TCTTTCTACATTAAACCCAGTTGCTTACGGTG(+AAAAAA)GATCCACATGTAAACTGTTTGGGGGCAAAAG NGG 10 8 17 8 24 0 100 5 10