Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145348_wg2z GCTTGAGGACATTGTTTGCTAGGTCTAGCCCG(+AAAAAA)GTACCTCCACATTCTGAAGGTGCTGCAATTG NGG 10 8 17 8 24 0 100 5 10
    240606145347_a3fe AAGTGAACCCCGCTGTGATGTGAAAATTTCAG(+AAAAAA)GATCCACACCAATGAACAGAATAAGCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606145345_yk6m GATCTTCTAGGCTTATTCTGTTCATTGGTGTG(+AAAAAA)GATCCTGAAATTTTCACATCACAGCGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606145342_rt97 ACTGTCATATTTATACATGGCTTTTCCCCTAG(+AAAAAA)GATCCTCAATCCTTCAGACCTAAACACAATT NGG 10 8 17 8 24 0 100 5 10
    240606145341_4hbv CCTGAAATTGTGTTTAGGTCTGAAGGATTGAG(+AAAAAA)GATCCTAGGGGAAAAGCCATGTATAAATATG NGG 10 8 17 8 24 0 100 5 10
    240606145340_n17z GGCCCCAGGTGTGCCAGCAGCTCAGGACATGG(+AAAAAA)GATCCAGTGGGCTCTCCTTCCTCTGCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606145338_bc5l TGGAGAACCTGCAGAGGAAGGAGAGCCCACTG(+AAAAAA)GATCCCATGTCCTGAGCTGCTGGCACACCTG NGG 10 8 17 8 24 0 100 5 10
    240606145336_u3hu CTCTATTCCATACTATATCCGTTGTGTGGCTG(+AAAAAA)GTACCTTAGTGGGGAAGGATGTCTCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606145335_cp3u GGGCCCATGCTGAGACATCCTTCCCCACTAAG(+AAAAAA)GTACCAGCCACACAACGGATATAGTATGGAA NGG 10 8 17 8 24 0 100 5 10
    240606145334_pqvq TTTTTTTCTAGCATGCTTAGGACCCTGAGTTG(+AAAAAA)GATCCCCAGCACCAGAGGGCAACAGGGAGAG NGG 10 8 17 8 24 0 100 5 10