Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145245_9ztx GGGGAAACATGCTCTCCCTGAAATCCCTGAAG(+AAAAAA)GATCCCCTTACCCTTGGTCCCATGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606145243_hd13 GCACAAAAAGAGTGTCGCTCAAGTTTAGTGGG(+AAAAAA)GTACCACACAAGGAATGAACACATTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606145242_ti5l CTCATAGCCTCAATGTGTTCATTCCTTGTGTG(+AAAAAA)GTACCCCACTAAACTTGAGCGACACTCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606145241_jrcn CATTTCCCTTCTGGGTACAAGGGCCTGTTTTG(+AAAAAA)GATCCTAGAATATCAAATATATACCACACCA NGG 10 8 17 8 24 0 100 5 10
    240606145240_bcpp GAGTATGGTGTGGTATATATTTGATATTCTAG(+AAAAAA)GATCCAAAACAGGCCCTTGTACCCAGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606145238_8x55 GCCCCCACAACCTAGCCCAAGAACCAGCCTGG(+AAAAAA)GTACCCATGGATTATATTGGGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606145235_d4r3 CTCTCTGCTGCCCCTCCCAATATAATCCATGG(+AAAAAA)GTACCCAGGCTGGTTCTTGGGCTAGGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606145233_iee5 CAGGGAATGAGATTGGCCCTTTCTCTGCCAGG(+AAAAAA)GTACCACAGGGACACATGCCGGGTAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606145231_tss5 CATACTCAGGCTACCCGGCATGTGTCCCTGTG(+AAAAAA)GTACCCTGGCAGAGAAAGGGCCAATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240606145229_pjln TTGGTGAGGGTCCTGAGGTGCTCTGGCCACAG(+AAAAAA)GTACCATCCATAGCTCCAACTCATGTGACCA NGG 10 8 17 8 24 0 100 5 10