Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145133_ynux AGGGGCTAGTTCCGACCAGGGGTCTGCCAAGG(+AAAAAA)GTACCCATGCCTTGCAGGTTAGAGTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606145131_s26m AACTGGGTTTGCTGAATATACCTGAAGGCCTG(+AAAAAA)GTACCTGGGAGGTAGAGGTGGAGGCGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606145129_no92 CTGATCTTCCCGCCTCCACCTCTACCTCCCAG(+AAAAAA)GTACCAGGCCTTCAGGTATATTCAGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240606145127_gxn3 ACCACATAGTGGCTCACAACCATCTGCAATGG(+AAAAAA)GATCCGATGCTCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606145126_oqa1 GCTGTCTTCAGACACACCAGAAGAGAGCATCG(+AAAAAA)GATCCCATTGCAGATGGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606145125_thyu GCTTGTTTTCTCTTTGTAAAGGACAAACTATG(+AAAAAA)GTACCAGAATCTGCCAAAGATCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606145124_8hgo TGAGATGAGAAGGGGATCTTTGGCAGATTCTG(+AAAAAA)GTACCATAGTTTGTCCTTTACAAAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606145121_pi4p CTTGCTTAGAGCCAGAAGGGATGATGTCCTGG(+AAAAAA)GATCCAAGACCAGAGGGACAGCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606145120_f8pd CTCCCCTCAGTGATGCTGTCCCTCTGGTCTTG(+AAAAAA)GATCCCAGGACATCATCCCTTCTGGCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240606145119_lqyw GTACATGAGTGTGGAAGTCTGAGCTATATTTG(+AAAAAA)GTACCCGATAGGATACTTTAGGTATCAGGAT NGG 10 8 17 8 24 0 100 5 10