Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145150_e7wv TTGTCCCCAGTTCTTTTTTTTTTTTGATGGAG(+AAAAAA)GTACCTGGGACTATGGGAGTACTTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240606145147_z3x7 GGGAAAATCTAAAGGAGACCCCACGTGCCCAG(+AAAAAA)GATCCGGGAACCAACCTTCCACACCACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606145145_44xl GGGGCTGGAGTGGTGTGGAAGGTTGGTTCCCG(+AAAAAA)GATCCTGGGCACGTGGGGTCTCCTTTAGATT NGG 10 8 17 8 24 0 100 5 10
    240606145144_jg55 GTCTCTTAAGGGGCAGCCCTAGGGTAGGATAG(+AAAAAA)GATCCTACCTCTCGCCAGCATCCCCCATGGA NGG 10 8 17 8 24 0 100 5 10
    240606145143_thft GCTGCTCCATGGGGGATGCTGGCGAGAGGTAG(+AAAAAA)GATCCTATCCTACCCTAGGGCTGCCCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606145141_ytyj TTGACTGTGGAGTCTCTTTTTTAAAGACACTG(+AAAAAA)GTACCAGGACTGACTGTCTCGACCTAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606145139_9pl2 CGTGCATTCTTAGGTCGAGACAGTCAGTCCTG(+AAAAAA)GTACCAGTGTCTTTAAAAAAGAGACTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606145138_7j0q GGCTTCTTCCTGCCTGTGGACGTTGCCAGTCG(+AAAAAA)GTACCTGTACTCCTCGTCTACTTCCGGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606145137_bs6e CATTCATAACCGGAAGTAGACGAGGAGTACAG(+AAAAAA)GTACCGACTGGCAACGTCCACAGGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606145135_5icm TATTTCTTTGCACTCTAACCTGCAAGGCATGG(+AAAAAA)GTACCCTTGGCAGACCCCTGGTCGGAACTAG NGG 10 8 17 8 24 0 100 5 10