Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145059_67cp CAGTCCCTCATCCCTGGGTCAATGGATCGATG(+AAAAAA)GATCCCTGGAAATTGGAATGGATGTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240606145057_i4ox AGTTCCCAAATCTCCAGTCTAAATCCTTTTTG(+AAAAAA)GATCCCACAGGACCCCACCACACTCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606145056_0waz GGGGGGGAAAGGAGTGTGGTGGGGTCCTGTGG(+AAAAAA)GATCCAAAAAGGATTTAGACTGGAGATTTGG NGG 10 8 17 8 24 0 100 5 10
    240606145054_3yle TGCTGAGACATGCTGGGCATGGCAGCTGTGAG(+AAAAAA)GTACCTCCCACATCTGCTTTCTGGCTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606145053_njbj AAGTCAGCACAGCCAGAAAGCAGATGTGGGAG(+AAAAAA)GTACCTCACAGCTGCCATGCCCAGCATGTCT NGG 10 8 17 8 24 0 100 5 10
    240606145051_ws72 CTCTTGTAGAAGCCAGTCAGGAACTGAGCCTG(+AAAAAA)GATCCTGTCCACATAAGCCTGCCCAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606145050_snsk TAGTGCTGCTCTGGGCAGGCTTATGTGGACAG(+AAAAAA)GATCCAGGCTCAGTTCCTGACTGGCTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606145047_zc82 GCGCCCCCTGGCGGGACCCTTGACCCCCAAAG(+AAAAAA)GATCCACCAGAGCAGTTGCTGTGCTCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606145046_3ddv ATATTCTAGAGAGCACAGCAACTGCTCTGGTG(+AAAAAA)GATCCTTTGGGGGTCAAGGGTCCCGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606145043_mkvx GCTCCATTATTCCCCACACAAGACTCCCCTGG(+AAAAAA)GTACCACGGAGAACTCCTCTAAAACCTCTTA NGG 10 8 17 8 24 0 100 5 10