Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145009_vtx9 CTATGGACGGCTCGGGGCACAGGCTAGGGAGG(+AAAAAA)GATCCAGAGAAGGCACAGACCAAATTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606145007_xubv CACCCACTTGAGGTCTCCCGACATTCACCCTG(+AAAAAA)GTACCACACCACTTGATCTCCAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606145006_ix21 GGGCCAGATTGCTCTGGAGATCAAGTGGTGTG(+AAAAAA)GTACCAGGGTGAATGTCGGGAGACCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606145004_n73h TCGCCAGTTCTGTTTGGAGTCCGTGTAGGTGG(+AAAAAA)GATCCTGCTGTGTGTAGCACTGGCCTGGACA NGG 10 8 17 8 24 0 100 5 10
    240606145003_80qe GGAGATGTCCAGGCCAGTGCTACACACAGCAG(+AAAAAA)GATCCCACCTACACGGACTCCAAACAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606145001_z2le TGTAGGGGGTTGAGAGGAAGGGAATGAGCCAG(+AAAAAA)GTACCCTGCATTCCAAGGGAGTTTCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606144959_48xo CTTTGCACACTGAAACTCCCTTGGAATGCAGG(+AAAAAA)GTACCTGGCTCATTCCCTTCCTCTCAACCCC NGG 10 8 17 8 24 0 100 5 10
    240606144957_ubif GAAGGTTTAGAGAATCCGATGGGCGCTGCCTG(+AAAAAA)GATCCCGGCATAGCCCCAGACACTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606144955_75d0 ATAAAGGAACCAAGTGTCTGGGGCTATGCCGG(+AAAAAA)GATCCAGGCAGCGCCCATCGGATTCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240606144954_bpwm GCTTTAGATAAGTCCCTGACACCAAGCTTCAG(+AAAAAA)GATCCAAGTGTTAACAGGAAAGAGCTGAAAT NGG 10 8 17 8 24 0 100 5 10