Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144937_gz1c ACAAATCAAGGGGAAAGTCTGACAGTAAAAAG(+AAAAAA)GATCCTATTGATTTGTTCACAAAAAGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606144936_vv1e TCACAGGTCTTTGTGAGGACTCCAGATCTTGG(+AAAAAA)GATCCACCACTTAGCATTATAATAGATAGCA NGG 10 8 17 8 24 0 100 5 10
    240606144934_abrg GGTCTTGCTATCTATTATAATGCTAAGTGGTG(+AAAAAA)GATCCCAAGATCTGGAGTCCTCACAAAGACC NGG 10 8 17 8 24 0 100 5 10
    240606144932_9xx9 ACTTCAAAAGTTGGTTTTTCTCCTTCTACTGG(+AAAAAA)GATCCAAGGATACAACTCAGGTTATCAGACT NGG 10 8 17 8 24 0 100 5 10
    240606144930_16ag GAACTAGTCTGATAACCTGAGTTGTATCCTTG(+AAAAAA)GATCCCAGTAGAAGGAGAAAAACCAACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606144929_ecc1 ATTGGAAGAGCAACATGGGCCACCCCTGCTGG(+AAAAAA)GTACCCAACTTATGAGCAAGAGAATGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606144927_5af3 TGGAAGCTTCCATTCTCTTGCTCATAAGTTGG(+AAAAAA)GTACCCAGCAGGGGTGGCCCATGTTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606144925_dc3j GCTCTGCGCACAGTTTGTTCACTAGAGGCCTG(+AAAAAA)GTACCTGTCTTCTCTCAGGCCTCTGCACTCA NGG 10 8 17 8 24 0 100 5 10
    240606144924_26xi CCGTGTGAGTGCAGAGGCCTGAGAGAAGACAG(+AAAAAA)GTACCAGGCCTCTAGTGAACAAACTGTGCGC NGG 10 8 17 8 24 0 100 5 10
    240606144922_bie1 TGGATCAGGAGCCATATTAAGATGACTTTGGG(+AAAAAA)GATCCCAGCAGTCCCTACGTGATCACATAGC NGG 10 8 17 8 24 0 100 5 10