Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150159_xobc TTTGTATGGGGACTTGAGTAAATAAATAAAGG(+AAAAAA)GATCCCACCTGAGAGAGCCACATCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240606150158_7jtq TTATTATTCATTTAACCATCCATCCACACAAG(+AAAAAA)GTACCTTCTTGAAACCTCTGGGCAGTCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606150157_1ey5 CAAATGTGAGACTGCCCAGAGGTTTCAAGAAG(+AAAAAA)GTACCTTGTGTGGATGGATGGTTAAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240606150155_meh0 AAGGAACATTTAACATCAAGATTTAAACAGTG(+AAAAAA)GTACCACTTGGGTGGCAAACTGGTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606150154_0orb AGATCTTTAAGAACCAGTTTGCCACCCAAGTG(+AAAAAA)GTACCACTGTTTAAATCTTGATGTTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606150152_ooag AAACATTCCATGTTCCATTGTCGCTCCTCCGG(+AAAAAA)GTACCCAGTTGGGACCCTGGTGCCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240606150150_var5 CACTGAAATGCTGGCACCAGGGTCCCAACTGG(+AAAAAA)GTACCCGGAGGAGCGACAATGGAACATGGAA NGG 10 8 17 8 24 0 100 5 10
    240606150148_4bl2 CAGTCATGCCCAGCTTTCATAGGGGTTCTGGG(+AAAAAA)GATCCAAACTTAGGTCTTCACACATGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606150147_sdyp ACGTGGTGAGCATGTGTGAAGACCTAAGTTTG(+AAAAAA)GATCCCCAGAACCCCTATGAAAGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606150146_0u7o TGGACAGCCATGTTGCTATTTGGCATGAAGAG(+AAAAAA)GATCCCACTCAAAGATCCAAGGGAGGAATCT NGG 10 8 17 8 24 0 100 5 10