Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144836_7qy8 ACCTAGAGTACAGATCCCCTGGGAATTTGTAG(+AAAAAA)GATCCCTAGAAGTTGTCTGTTAGGCAACTGT NGG 10 8 17 8 24 0 100 5 10
    240606144833_z8py GGATGGACTATCCAGAGACTACCCCACCCAGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606144831_q511 AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCTGGGTGGGGTAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606144830_664e CATCCTTTATAAGTGGACAATATGTGTATGTG(+AAAAAA)GATCCCGCAAGGCAGGCCAAGTCTTCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606144829_do6o AATGAGAACTGAAGACTTGGCCTGCCTTGCGG(+AAAAAA)GATCCACATACACATATTGTCCACTTATAAA NGG 10 8 17 8 24 0 100 5 10
    240606144827_vxg9 GGTCCCAGCTACGTGGGCAGCTAAGGCAGGAG(+AAAAAA)GATCCCATTAGCCCATAAGCTCAGGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240606144825_witp CCCAGTATAGCCCTGAGCTTATGGGCTAATGG(+AAAAAA)GATCCTCCTGCCTTAGCTGCCCACGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606144824_gt1v AGCGCCAGCCCTAGGAACCCCAGAAAAGAGTG(+AAAAAA)GTACCTAAGGCTGAAATTGTTTTGCTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606144822_u690 CCCTGGCAACAGCAAAACAATTTCAGCCTTAG(+AAAAAA)GTACCACTCTTTTCTGGGGTTCCTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606144821_it54 TCTAAAATTTAAGCCCTTTAAATATCACAAAG(+AAAAAA)GTACCCTTGTTGGCCTTACAAAAGATAGCTA NGG 10 8 17 8 24 0 100 5 10