Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144731_zwec CCCAGCCAAGGAGCACGAGTGAGAACGCTCGG(+AAAAAA)GATCCGAGAGAAAAACAGGAGCAGCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240606144730_1io2 TGGGAGGTGCTGCTGCTCCTGTTTTTCTCTCG(+AAAAAA)GATCCCGAGCGTTCTCACTCGTGCTCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606144729_gp87 AACCAGGCTGGGATATGACACCTCCAGTGGAG(+AAAAAA)GATCCTTCTACACACAACACAGGTTTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606144727_fgs1 GTTTTCTTCTAAACCTGTGTTGTGTGTAGAAG(+AAAAAA)GATCCTCCACTGGAGGTGTCATATCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606144726_n8s8 CTGGAAAGACAAGCGGTCAAAGCTGTGGCTTG(+AAAAAA)GATCCACACTATGGGCATTTTGGTGATTCTT NGG 10 8 17 8 24 0 100 5 10
    240606144725_0wav AGGACAAGAATCACCAAAATGCCCATAGTGTG(+AAAAAA)GATCCAAGCCACAGCTTTGACCGCTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606144723_t0m2 GGAGGTGACCCTTAAGGATCTAGATACTGATG(+AAAAAA)GTACCCAGCTGGCAAGTGGCACAGACCACTG NGG 10 8 17 8 24 0 100 5 10
    240606144722_esr4 GAAGCCAGTGGTCTGTGCCACTTGCCAGCTGG(+AAAAAA)GTACCATCAGTATCTAGATCCTTAAGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606144720_8d9l GCTCCCCCAATGCAGGGTAGGGTGCCAGGTGG(+AAAAAA)GATCCAAGACAGCCTTATGTCCTGTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606144719_dpm0 GGTTCATGTTTACAGGACATAAGGCTGTCTTG(+AAAAAA)GATCCCACCTGGCACCCTACCCTGCATTGGG NGG 10 8 17 8 24 0 100 5 10