Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145905_90lj GAGTTCATCTTATATTTATATTTAATTCACAG(+AAAAAA)GTACCAAAAGAATATCTATAATGAATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606145904_sljx ATACCCCTTGGCTATGCCCACAAATCCATAAG(+AAAAAA)GATCCTGACTTAGAAAGAAAAACAGCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606145903_2a07 TTGAATTTGTGCTGTTTTTCTTTCTAAGTCAG(+AAAAAA)GATCCTTATGGATTTGTGGGCATAGCCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606145902_6uol AGCAAGGCTCTCTCTTCTGGGCAAGGGCAATG(+AAAAAA)GATCCTCAGAGGCCCCGGCAGAAGCCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240606145900_yhpp AGAGAAGCTAGGCTTCTGCCGGGGCCTCTGAG(+AAAAAA)GATCCATTGCCCTTGCCCAGAAGAGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606145859_qzmb GTCTTCACACTCTCCTGTATGTATCTGTATTG(+AAAAAA)GTACCAATGAAATACAGTTCAGCATTTAATA NGG 10 8 17 8 24 0 100 5 10
    240606145858_k1wn TAGCATATTAAATGCTGAACTGTATTTCATTG(+AAAAAA)GTACCAATACAGATACATACAGGAGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606145857_1k8f TCTCCCAACTATTCATAATTCTAGTTCAAGTG(+AAAAAA)GATCCAGCACCCTCTTCTAACTGATACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606145855_8fwm GTACTGGCAGTATCAGTTAGAAGAGGGTGCTG(+AAAAAA)GATCCACTTGAACTAGAATTATGAATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606145852_mvfb TTTGTAGACCAGGCTGGCCTTGAAGTCCCAGG(+AAAAAA)GATCCTGTCTGTGCCTCTCACATGTGTTAAG NGG 10 8 17 8 24 0 100 5 10