Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144646_8jsc AAGCTAAATGTGTGCATACTCTATGTGGAGTG(+AAAAAA)GATCCACTCCGAACTGTACACCCTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606144645_tod6 GCACATGTATTTAGGGTGTACAGTTCGGAGTG(+AAAAAA)GATCCACTCCACATAGAGTATGCACACATTT NGG 10 8 17 8 24 0 100 5 10
    240606144643_33bf GGGCTCTGCTCAGCATTGTCTGAGGGGTTGGG(+AAAAAA)GTACCCACACAGGTGCTTGCTGCTTCCCACG NGG 10 8 17 8 24 0 100 5 10
    240606144641_uf9n AATGCCGTGGGAAGCAGCAAGCACCTGTGTGG(+AAAAAA)GTACCCCAACCCCTCAGACAATGCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606144639_ixwc CACCAAGCCTGATGACCTGAGTTAGACCTCGG(+AAAAAA)GATCCATGAGGTAGAAGAAGAGAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606144638_6rfs TAAAGGGCTCATTTCTCTTCTTCTACCTCATG(+AAAAAA)GATCCCGAGGTCTAACTCAGGTCATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606144636_lpuk GAGGTTCTGGGTTCAATTCCCATCTGTAATGG(+AAAAAA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606144635_02zp GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCCATTACAGATGGGAATTGAACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606144633_urmn GCAGGAAGTCCTGAGGTTAGGGACTGGCTATG(+AAAAAA)GTACCCAGGACCCGGTCAGTAGTACCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606144631_u3wn TGAGACAGCAGGTACTACTGACCGGGTCCTGG(+AAAAAA)GTACCATAGCCAGTCCCTAACCTCAGGACTT NGG 10 8 17 8 24 0 100 5 10