Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144703_1vip TCAGGTCTACATGGAACCTAGAATTTCAGCTG(+AAAAAA)GATCCTGAGAGCTGGTCTTCAACAAGCGAAG NGG 10 8 17 8 24 0 100 5 10
    240606144701_5e5b CTGACCTTCGCTTGTTGAAGACCAGCTCTCAG(+AAAAAA)GATCCAGCTGAAATTCTAGGTTCCATGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606144700_kibh CAGGTGTGGTGGGAATTGTAATCTCAGGACTG(+AAAAAA)GATCCCACCCAACTAAAGTTATTGTCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606144658_pekg GTTAAACACAGACAATAACTTTAGTTGGGTGG(+AAAAAA)GATCCAGTCCTGAGATTACAATTCCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606144657_a3dc TAGGGCTTCTCTCCAGTGTGAGTCCTTTCATG(+AAAAAA)GATCCGGAAAGAATGAGAGCAACTGAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606144655_21pm CACAAGGCCTTCAGTTGCTCTCATTCTTTCCG(+AAAAAA)GATCCATGAAAGGACTCACACTGGAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606144654_8zs0 ACCACATGGTGGCTCACAACCATCTGTGATGG(+AAAAAA)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606144653_3h02 CTTGTCTTCAGACACACCAGAAGAGGGCATTG(+AAAAAA)GATCCCATCACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606144649_y7d6 CTCATGGAAGCTATTTTGCTCTGTACCCCGGG(+AAAAAA)GATCCCCAGCATTAGGCCATTGGACCCCATC NGG 10 8 17 8 24 0 100 5 10
    240606144647_42jn AGAGAGATGGGGTCCAATGGCCTAATGCTGGG(+AAAAAA)GATCCCCGGGGTACAGAGCAAAATAGCTTCC NGG 10 8 17 8 24 0 100 5 10