Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144556_eaa1 TTCAGGGGACCAAGATGTTGGGCATAAATCAG(+AAAAAA)GATCCTACATGGTGCTGCCCTGCTCATAACT NGG 10 8 17 8 24 0 100 5 10
    240606144555_r8re TTCCCAGTTATGAGCAGGGCAGCACCATGTAG(+AAAAAA)GATCCTGATTTATGCCCAACATCTTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606144553_7us1 AGACCTCATTATTTATGTGTTTGTATGTGTGG(+AAAAAA)GTACCCAGAGACCAGAAGAGGAAGAGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606144551_rxs0 GGAGGTCAGACTCTTCCTCTTCTGGTCTCTGG(+AAAAAA)GTACCCACACATACAAACACATAAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240606144548_pytx GCTCACAAGCATCTGTAATTCCAGTTCCAGGG(+AAAAAA)GATCCAACACCCTCTTCTGGTTGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606144547_jrp6 TTCTCATGGAGACCAACCAGAAGAGGGTGTTG(+AAAAAA)GATCCCCTGGAACTGGAATTACAGATGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606144545_p5g0 AAGATGCCATGCACACTGTCAGAGGACAAAGG(+AAAAAA)GATCCTCAGTGTCACCCAGCTGCAGGTCGCT NGG 10 8 17 8 24 0 100 5 10
    240606144544_6m0a ACACCAGCGACCTGCAGCTGGGTGACACTGAG(+AAAAAA)GATCCCTTTGTCCTCTGACAGTGTGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606144543_63ln GAACTTGCTGCTAAGCCAGACAGCCTGAGTTG(+AAAAAA)GATCCCTGGGACTCATGGCCTGCTGCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240606144540_0lrt AAGACAGCCGGCAGCAGGCCATGAGTCCCAGG(+AAAAAA)GATCCAACTCAGGCTGTCTGGCTTAGCAGCA NGG 10 8 17 8 24 0 100 5 10