Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144523_hcx1 TGCTCAGTCTATGATGCCCTTGAGCTACCATG(+AAAAAA)GTACCTTGTGCAACATGGCTTGATTAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606144521_zyq4 CCCAGGAAGCTAATCAAGCCATGTTGCACAAG(+AAAAAA)GTACCATGGTAGCTCAAGGGCATCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240606144520_51b1 TAGTTCTAACTTAAACCACTTGGTGATTGGTG(+AAAAAA)GTACCGTCAGGTGCTGGGCTTCAAAAACTTG NGG 10 8 17 8 24 0 100 5 10
    240606144519_ll8x CTTGCCAAGTTTTTGAAGCCCAGCACCTGACG(+AAAAAA)GTACCACCAATCACCAAGTGGTTTAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606144517_lbct GTGTCAAATTCTGTGAACCAGGGCCAACACTG(+AAAAAA)GTACCAGCTTCACCCTGACCTGGGGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606144516_l0c4 GAGGGCAGTGGCCCCAGGTCAGGGTGAAGCTG(+AAAAAA)GTACCAGTGTTGGCCCTGGTTCACAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240606144515_j1mz TATAGCTAAGTTTACACCAACCAAATTATTTG(+AAAAAA)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606144514_rv9a TACCCCAGCATACCCAGTATTTACAAGAATTG(+AAAAAA)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10
    240606144512_2m4c CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+AAAAAA)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606144511_bvfy CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+AAAAAA)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10