Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144717_jljv TATGGATAAAATTGGTGCAAATTGGTCAAACG(+AAAAAA)GATCCAACAAACACTGATGTCCAAGCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240606144716_em6i CAATATGCCAGCTTGGACATCAGTGTTTGTTG(+AAAAAA)GATCCGTTTGACCAATTTGCACCAATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606144715_ca4h ATTCCAAGGCCAAGTCCAGTCTATGAAACAAG(+AAAAAA)GTACCATACCCAGTCTGAGCATGGGATGCTA NGG 10 8 17 8 24 0 100 5 10
    240606144713_t75i AAAACTAGCATCCCATGCTCAGACTGGGTATG(+AAAAAA)GTACCTTGTTTCATAGACTGGACTTGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606144712_c85w GTGCACCACGGGCCGGGCTTCATCTGTGATTG(+AAAAAA)GATCCAGGGAAGAATCAGCGTGAAGTATATT NGG 10 8 17 8 24 0 100 5 10
    240606144709_yrtn TGCTGAATATACTTCACGCTGATTCTTCCCTG(+AAAAAA)GATCCAATCACAGATGAAGCCCGGCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240606144708_77vz AAACATGGGGACTAATAACTTAGAGACAGTTG(+AAAAAA)GTACCGTGTCTAATTTCTGCCTAAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606144707_u1by GAAAGCATCTCTTTAGGCAGAAATTAGACACG(+AAAAAA)GTACCAACTGTCTCTAAGTTATTAGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606144705_4r00 AAAAAATGGGCTATGAAATTATTTTACATCTG(+AAAAAA)GATCCCTGTATTATAAAGTCTGTCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606144704_88fk ACGAAGTTTGGGGACAGACTTTATAATACAGG(+AAAAAA)GATCCAGATGTAAAATAATTTCATAGCCCAT NGG 10 8 17 8 24 0 100 5 10