Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144746_3txl GGATGATTCTACCTCCAGACTTCCTCTTCCTG(+AAAAAA)GATCCATAGCAATATGCAGCTATGGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606144745_ep4m TTTATACAAGGCCATAGCTGCATATTGCTATG(+AAAAAA)GATCCAGGAAGAGGAAGTCTGGAGGTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606144744_dotl TTCCCTTCAGTATCTGCTCAGGCAGAAGATGG(+AAAAAA)GTACCCCCACCTCCACCATCATCTTCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606144742_u4p1 CGTGGGAGCAGAAGATGATGGTGGAGGTGGGG(+AAAAAA)GTACCCATCTTCTGCCTGAGCAGATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240606144740_vxbp ATGTTCTGGGATGCCTCCCCTGAAGCCAATGG(+AAAAAA)GTACCAACATCACCAGGAGGAGGAAGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606144739_by39 GACTCTTTTGCTTCCTCCTCCTGGTGATGTTG(+AAAAAA)GTACCCATTGGCTTCAGGGGAGGCATCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606144737_4kcc AGTCTGTGAGGGAGCCTGACAGGGAGCACCAG(+AAAAAA)GATCCTTCACCTCCCCACTGCCGTGTGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606144736_xb5e GTGTGTAGACACACGGCAGTGGGGAGGTGAAG(+AAAAAA)GATCCTGGTGCTCCCTGTCAGGCTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606144735_bwf3 AAGGTGAATCACAAGTATTGGAAACAGTTCAG(+AAAAAA)GATCCTGGCAGTAGCTTTCTCAGAGCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606144733_4klb TGGGAAGGCTGCTCTGAGAAAGCTACTGCCAG(+AAAAAA)GATCCTGAACTGTTTCCAATACTTGTGATTC NGG 10 8 17 8 24 0 100 5 10