Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150004_97cy TGGAGCTACCTGTATATGAAGGTAGGATGAGG(+AAAAAA)GTACCAGGGAAAGTTGATCAGAGCTACAGCC NGG 10 8 17 8 24 0 100 5 10
    240606150002_vcto CATCCCAAAGTTGGCCTTTGTCCTTCCCCATG(+AAAAAA)GATCCCTAAAAACAAACAAACAAATAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606150000_yqob TTTATTTTTTTATTTGTTTGTTTGTTTTTAGG(+AAAAAA)GATCCATGGGGAAGGACAAAGGCCAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606145959_7oxp AGGTTCCAGACTTCTCCCAGCCACCGTGGCAG(+AAAAAA)GTACCCCTCATGTCAGGGACTGCCTCGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606145957_pkz3 AAAGCAGCACGAGGCAGTCCCTGACATGAGGG(+AAAAAA)GTACCTGCCACGGTGGCTGGGAGAAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606145955_a0pg TAGTTAGCGATGAGGAGGAAGACACTTGAATG(+AAAAAA)GATCCCCACCCTAGGATGCTGCTCTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606145953_f48z GGAAGGCAAGAAGAGCAGCATCCTAGGGTGGG(+AAAAAA)GATCCATTCAAGTGTCTTCCTCCTCATCGCT NGG 10 8 17 8 24 0 100 5 10
    240606145952_5xv0 TATTGATTCTCTTAGAACTTTGTCAGTGGATG(+AAAAAA)GTACCTTGCCTTTGCAGGTTGTATGTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606145950_k5eb ATCTAAGGTCACATACAACCTGCAAAGGCAAG(+AAAAAA)GTACCATCCACTGACAAAGTTCTAAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606145949_afsa CAAACAGAGACAAGCTTCTCTGAATGTGGGTG(+AAAAAA)GTACCATTCCCATAGGCTTTGGCCAAGGAGA NGG 10 8 17 8 24 0 100 5 10