Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144920_b0w2 CAGGAGCTATGTGATCACGTAGGGACTGCTGG(+AAAAAA)GATCCCCAAAGTCATCTTAATATGGCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606144919_gd3a ATGATTACTAGCACTAGAAGCCAACAGCAAAG(+AAAAAA)GATCCCGCGCGCAGTTGCTCTTGTTTAATCC NGG 10 8 17 8 24 0 100 5 10
    240606144917_24pa AACCTGGATTAAACAAGAGCAACTGCGCGCGG(+AAAAAA)GATCCTTTGCTGTTGGCTTCTAGTGCTAGTA NGG 10 8 17 8 24 0 100 5 10
    240606144915_ni0z TGGTCCATTTAAAGTTTCCCAGCTATGGGGTG(+AAAAAA)GTACCAGTTCTGGACAAGTGCCACTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240606144914_m074 TACTATGATGCAGTGGCACTTGTCCAGAACTG(+AAAAAA)GTACCACCCCATAGCTGGGAAACTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606144912_91zg GGGCGGGAGTAAGGAAATGCAGGAGTTGACCG(+AAAAAA)GATCCTTGTTCCTCACCTTGTACACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240606144911_78op AGTTTAAGTGAGTGTACAAGGTGAGGAACAAG(+AAAAAA)GATCCGGTCAACTCCTGCATTTCCTTACTCC NGG 10 8 17 8 24 0 100 5 10
    240606144909_mjjt CATGCTATATGTATACTTCTGTTTTCTGTTGG(+AAAAAA)GTACCTTGCAATGCATTCTATGCCTCATTAA NGG 10 8 17 8 24 0 100 5 10
    240606144908_y6r5 TCCATTTAATGAGGCATAGAATGCATTGCAAG(+AAAAAA)GTACCCAACAGAAAACAGAAGTATACATATA NGG 10 8 17 8 24 0 100 5 10
    240606144906_yewg CAAGAAAATATCATCCTGAATGAAGTATCCTG(+AAAAAA)GATCCAAAAGGACATGCATGGTGCTTACTCA NGG 10 8 17 8 24 0 100 5 10