Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150250_2ei0 GGAGAAAATGTAGATGGCCACCACTCTCTAGG(+AAAAAA)GTACCACACTTGAGAGGAATTGATTTGACCC NGG 10 8 17 8 24 0 100 5 10
    240606150247_ue70 GGCTCCGATTCCTTCACATTCCAGCTGGCCCG(+AAAAAA)GATCCCTGTGTGGCCATAAGGCCTTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606150245_ozkx GTGACGAGGGCAAGGCCTTATGGCCACACAGG(+AAAAAA)GATCCGGGCCAGCTGGAATGTGAAGGAATCG NGG 10 8 17 8 24 0 100 5 10
    240606150243_glxq GGGGCACATCTGTGGAGAGGCAGAAGCCAAGG(+AAAAAA)GATCCTGGCTGTGCCCTATGATTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606150241_69nj GTCAAGATCTGGGAATCATAGGGCACAGCCAG(+AAAAAA)GATCCCTTGGCTTCTGCCTCTCCACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240606150239_gipi TTTTTACAAGGCTTATAAATCCTTTAGTGTGG(+AAAAAA)GATCCTGTCCCACCATCGGCAGCTGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606150238_oahl TGTCCCAACCACAGCTGCCGATGGTGGGACAG(+AAAAAA)GATCCCACACTAAAGGATTTATAAGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606150237_7ze7 ACCAATGGCACCAGGGTCAGAAGACTGACTTG(+AAAAAA)GATCCGTCACAGAACCCTTTTCAGAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606150236_ukft GAGATGCAGCCTCTGAAAAGGGTTCTGTGACG(+AAAAAA)GATCCAAGTCAGTCTTCTGACCCTGGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606150234_k8bd GATAGGAGGCTATGAGGCCTCCTTAGGTCAGG(+AAAAAA)GATCCCAGCAAGAGGCTGGCTCGAGTTGTGG NGG 10 8 17 8 24 0 100 5 10