Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145207_2vg1 ACATCCTCAGCAGGGGGCCAACCTCCAAGGAG(+AAAAAA)GATCCCTAAGCCTCCCATAGCAGTCGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606145205_rljs GCCCACGGAGGGCCTTTATTCATTGTCCACAG(+AAAAAA)GTACCCTGGAGCTGTGGCCTCCAGGATAGAG NGG 10 8 17 8 24 0 100 5 10
    240606145203_jsxu GGTATCTCTATCCTGGAGGCCACAGCTCCAGG(+AAAAAA)GTACCTGTGGACAATGAATAAAGGCCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240606145201_qnd7 ATTCACCTGCTCTTTGGGCAAACATTCATTGG(+AAAAAA)GTACCACAACACCAAACCTCCCGAGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606145200_u61f TAGTTGCCTGTCTCGGGAGGTTTGGTGTTGTG(+AAAAAA)GTACCCAATGAATGTTTGCCCAAAGAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606145159_3kbq ATATCGGCTGAATACCGGCTGAACTAAAACTG(+AAAAAA)GTACCCGCTGCCTTCAGTCCAGTGGGTCACA NGG 10 8 17 8 24 0 100 5 10
    240606145157_gdym CTGCCTGTGACCCACTGGACTGAAGGCAGCGG(+AAAAAA)GTACCAGTTTTAGTTCAGCCGGTATTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606145156_wzaq AAAGACTCCCACCCCTGGCAGGAAGCAGGAAG(+AAAAAA)GATCCGCCAGGCCTGGAGCATTCCTTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606145154_jzfg ACTGTGGAGGAAGGAATGCTCCAGGCCTGGCG(+AAAAAA)GATCCTTCCTGCTTCCTGCCAGGGGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606145152_p4up AACAATATAAAAAAGTACTCCCATAGTCCCAG(+AAAAAA)GTACCTCCATCAAAAAAAAAAAAGAACTGGG NGG 10 8 17 8 24 0 100 5 10