Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145432_rmcc AGGCCCCCGGGCAAGCAGGGGGTTGGGGGGAG(+AAAAAA)GATCCCGGCAGGCTCAGGTTTTACCCTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606145430_kdkw AGGCAGACAGCACCCTGTGGGAGATCAGATGG(+AAAAAA)GATCCTGAGCCCCTTGTATGGCCGTGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606145429_nqac AGAGGCCACCCACGGCCATACAAGGGGCTCAG(+AAAAAA)GATCCCATCTGATCTCCCACAGGGTGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606145426_cpr7 AGGGGCCAACAACACAGTGTAAAGAGACCCAG(+AAAAAA)GATCCCTCTGTGATCCCCACCACAATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606145424_1ykg TCAGTACTCCATTGTGGTGGGGATCACAGAGG(+AAAAAA)GATCCTGGGTCTCTTTACACTGTGTTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606145423_r0qn ACACACATAGCTAGTTTTGCTTAAAATATTTG(+AAAAAA)GATCCCGAGTTTCCATTTTCCTAAAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606145421_k88y GGTTTCTCTTTTTTAGGAAAATGGAAACTCGG(+AAAAAA)GATCCAAATATTTTAAGCAAAACTAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606145420_t0u5 ACACACATAGCTAGTTTTGCTTAAAATATTTG(+AAAAAA)GATCCCGAGTTTCCATTTTCCTAAAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606145418_bfj7 GGTTTCTCTTTTTTAGGAAAATGGAAACTCGG(+AAAAAA)GATCCAAATATTTTAAGCAAAACTAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606145417_lbny GTGCTTAAGTAGCTAGATTCAAATCAAACATG(+AAAAAA)GATCCAAAAAAGTCTATTACCAACCAAAGAG NGG 10 8 17 8 24 0 100 5 10