Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145402_usrw TTTCACTTTTGCCCCCAAACAGTTTACATGTG(+AAAAAA)GATCCACCGTAAGCAACTGGGTTTAATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606145401_ykej CACTCACACTGGGAGGCATTGTTAAGTGAACG(+AAAAAA)GTACCGCAGGCTCAGATTTGAGCACTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606145359_pc9w GGTAGACAGAAGTGCTCAAATCTGAGCCTGCG(+AAAAAA)GTACCGTTCACTTAACAATGCCTCCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606145357_9arn AAATCCCAGCACTTGGGAGGAAGAGGCAGGAG(+AAAAAA)GATCCGAGTCTACATAGCCAGCTTGGTGTAC NGG 10 8 17 8 24 0 100 5 10
    240606145356_sycx ATTATGTACACCAAGCTGGCTATGTAGACTCG(+AAAAAA)GATCCTCCTGCCTCTTCCTCCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606145355_yior AATTGATTTTTACTCTTTACTATCCAAGTTTG(+AAAAAA)GATCCATTCAGAAGAGCTGGTTTTAAATTCT NGG 10 8 17 8 24 0 100 5 10
    240606145354_rlc8 CGAATAGAATTTAAAACCAGCTCTTCTGAATG(+AAAAAA)GATCCAAACTTGGATAGTAAAGAGTAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606145353_ciw7 AAGTGCACCTTTGTTATATACTTATTATATTG(+AAAAAA)GTACCAAATACAGAAGAAAACTATAGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606145352_t2w9 TATCACAGAACTATAGTTTTCTTCTGTATTTG(+AAAAAA)GTACCAATATAATAAGTATATAACAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606145350_r0s5 GCAAGCAATTGCAGCACCTTCAGAATGTGGAG(+AAAAAA)GTACCGGGCTAGACCTAGCAAACAATGTCCT NGG 10 8 17 8 24 0 100 5 10