Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144334_a7up GGACACACCTCAGGATCATTATCCCAGGAATG(+AAAAAA)GTACCACCCACAGTGGGTGGGGCTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606144333_8qe9 TGGACGTGGGAGAGCCCCACCCACTGTGGGTG(+AAAAAA)GTACCATTCCTGGGATAATGATCCTGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606144331_tbwh GGCCCTGCTGGCCTAGCGCTGTTTCTGTGGAG(+AAAAAA)GATCCTCCTGTCTCAGCCTTCTGAGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606144330_vugs CAAGCTCAGCACTCAGAAGGCTGAGACAGGAG(+AAAAAA)GATCCTCCACAGAAACAGCGCTAGGCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606144328_00sp GGTGGGAAGTCGAAGATGGGGAGTCTAAGAGG(+AAAAAA)GATCCTATAGGACCCGCCTGACCTGGGAATT NGG 10 8 17 8 24 0 100 5 10
    240606144326_b0ol AGGACAATTCCCAGGTCAGGCGGGTCCTATAG(+AAAAAA)GATCCCTCTTAGACTCCCCATCTTCGACTTC NGG 10 8 17 8 24 0 100 5 10
    240606144325_tfpt GGTTTGTCCCTGAATCCCAGCCCTCCGGGTTG(+AAAAAA)GATCCGATCCTGTCAGGAGCAGTGGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606144324_6qxy GGAGGCAGACTCCACTGCTCCTGACAGGATCG(+AAAAAA)GATCCAACCCGGAGGGCTGGGATTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606144322_4gmu TCCCCAGAGCCTGATACAGGTGACCTCGGAAG(+AAAAAA)GTACCAGCAGTAGGACCAGTGGACCGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606144320_ojp1 AAGCGGAGCACGGTCCACTGGTCCTACTGCTG(+AAAAAA)GTACCTTCCGAGGTCACCTGTATCAGGCTCT NGG 10 8 17 8 24 0 100 5 10