Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145604_9g30 CCTAGGCCCCATCCCCAAAGAGCTCAAGTGAG(+AAAAAA)GATCCAGAGGAAGCTTCAAGTTGTTGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606145603_d7ob CTATGCTACCCCACTTCCTGTATGCTCAACTG(+AAAAAA)GATCCTCAAGACCATTTCATCTCTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606145602_ondf CGTGGTGCAGCTAGAGATGAAATGGTCTTGAG(+AAAAAA)GATCCAGTTGAGCATACAGGAAGTGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606145600_ta0r TGACAGACTTGATGCTTTGAAATTGACCATTG(+AAAAAA)GTACCTCCTCCTTAAAAGAGAAGTGTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240606145558_2a4c CCTGAACACGACACTTCTCTTTTAAGGAGGAG(+AAAAAA)GTACCAATGGTCAATTTCAAAGCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606145557_ymmf CTGGGTGTGCAGGAATTCTGCCTGTGAAGTGG(+AAAAAA)GATCCACAGCATGTTCGACGGGTGGGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606145555_lagj GCCCAGTTCTCCCACCCGTCGAACATGCTGTG(+AAAAAA)GATCCCACTTCACAGGCAGAATTCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240606145554_t5je CTTGTTCTACCATCTCTCCTGGGTTCTTCATG(+AAAAAA)GTACCACCTCGTGTACAGCCACGCCAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240606145553_po2q CTGGTTTAACTGGCGTGGCTGTACACGAGGTG(+AAAAAA)GTACCATGAAGAACCCAGGAGAGATGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606145550_ucvm CTCCGTACACTGTGTGGGCAGTGTGGCCTTGG(+AAAAAA)GATCCCCACTGATTACCAGGGATGGATGCCT NGG 10 8 17 8 24 0 100 5 10